Real-time toxicity prediction of Aconitum stewing system using extractive electrospray ionization mass spectrometry

Publication date: Available online 11 September 2019Source: Acta Pharmaceutica Sinica BAuthor(s): Zi-Dong Qiu, Jin-Long Chen, Wen Zeng, Ying Ma, Tong Chen, Jin-Fu Tang, Chang-Jiang-Sheng Lai, Lu-Qi HuangAbstractDue to numerous obstacles such as complex matrices, real-time monitoring of complex reaction systems (e.g., medicinal herb stewing system) has always been a challenge though great values for safe and rational use of drugs. Herein, facilitated by the potential ability on the tolerance of complex matrix of extractive electrospray ionization mass spectrometry, a device was established to realize continuous sampling and real-time quantitative analysis of herb stewing system for the first time. A complete analytical strategy, including data acquisition, data mining, and data evaluation was proposed and implemented with overcoming the usual difficulties in real-time mass spectrometry quantification. The complex Fuzi (the lateral root of Aconitum)–meat stewing systems were real-timely monitored in 150 min by qualitative and quantitative analysis of the nine key alkaloids accurately. The results showed that the strategy worked perfectly and the toxicity of the systems were evaluated and predicated accordingly. Stewing with trotters effectively accelerate the detoxification of Fuzi soup and reduce the overall toxicity to 68%, which was recommended to be used practically for treating rheumatic arthritis and enhancing immunity. The established strategy was versatile, simple, an...
Source: Acta Pharmaceutica Sinica B - Category: Cancer & Oncology Source Type: research