Volume-CLEM: A Method for Correlative Light- and Electron-Microscopy in Three Dimensions.

Volume-CLEM: A Method for Correlative Light- and Electron-Microscopy in Three Dimensions.
Am J Physiol Lung Cell Mol Physiol. 2019 Sep 11;:
Authors: Hegermann J, Wrede C, Fassbender S, Schliep R, Ochs M, Knudsen L, Mühlfeld C
Abstract
Generation of 3D datasets from serial sections of tissues imaged by light microscopy (LM) allows identification of rare structures by morphology or fluorescent labeling. Here, we demonstrate a workflow for correlative light- and electron microscopy (EM) from three-dimensional (3D) LM to 3D EM, using the same sectioned material for both methods consecutively. The new approach is easy to reproduce in routine EM laboratories and applicable to a wide range of organs and research questions.
PMID: 31508976 [PubMed - as supplied by publisher]

Source: Am J Physiol Lung Ce... - September 10, 2019 Category: Respiratory Medicine Authors: Hegermann J, Wrede C, Fassbender S, Schliep R, Ochs M, Knudsen L, Mühlfeld C Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research