1p36 is a chromosomal site of genomic instability in cervical intraepithelial neoplasia.

1p36 is a chromosomal site of genomic instability in cervical intraepithelial neoplasia. Biotech Histochem. 2019 Sep 10;:1-8 Authors: Cortés-Gutiérrez EI, García-Vielma C, Dávila-Rodríguez MI, Sánchez-Dávila H, Fernández JL, Gosálvez J Abstract We investigated the association between progressive stages of cervical neoplasia and DNA damage in 1p36 DNA sequences of chromosome 1 in cervical epithelium using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH). We used a hospital based unmatched case control study of 29 women that were grouped according to disease stage and selected according to histological diagnosis: 10 with low grade squamous intraepithelial lesions (LG-SILs), 10 with high grade SILs (HG-SILs) and nine with no cervical lesions; the 1pter sequence was used as internal control. We found a significant increase in the number of patients with HG-SIL compared to patients with LG-SILs or with no cervical lesions. 1p36 Genomic instability was validated by DBD-FISH using neutral comets. Genetic instability at specific gene loci, such as 1p36, might be characteristic of cervical cancer progression. DBD-FISH appears to be a useful approach for detecting and comparing damage to specific chromosomal regions related to the progression of cervical cancer. PMID: 31502894 [PubMed - as supplied by publisher]
Source: Biotechnic and Histochemistry - Category: Research Authors: Tags: Biotech Histochem Source Type: research