Dipeptidyl peptidase ‐4 inhibitors and aerobic exercise synergistically protect against liver injury in ovariectomized rats

DPPi therapy and exercise improved liver fat metabolism, achieved significant beneficial metabolic effects and offer full protection against NAFLD by acting through controlling enzymes, autophagy and modulating glucose homeostasis and inflammatory process. Exercise maximizes the benefits provided by incretin ‐based therapy. AbstractMenopause increases the risk of non ‐alcoholic fatty liver disease (NAFLD). We investigated the effect of incretin and/ or exercise on the hepatic fat accumulation in ovariectomized rats. Rats were divided into five groups: Group 1: Control rats, Group 2: Ovariectomized rats, Group 3: Ovariectomized rats + Dipeptidyl peptidase‐4 inhibitor (DPPi) (30 mg/kg/day, orally), Group 4: Ovariectomized rats + swimming, and Group 5: Ovariectomized rats + swimming + DPPi. After 6 weeks, Alanine aminotransferase (ALT), glucose, insulin, HOMA IR (Homeostatic Model Assessment for Insulin Resistance), FFA (free fatty acids), Tumor necrosis factor alpha (TNFα), IL6, IL1B levels were measured in blood. The livers were collected for Hematoxylin and eosin (H&E) examination and evaluation of hepatic gene expression of SREBP (sterol regulatory element ‐binding protein1c), PPARα (peroxisome proliferator ‐activated receptor alpha), ACC 1 (acetyl‐CoA carboxylase), LC3 (microtubule‐associated protein 1 light chain 3), SIRT (sirtuin), hepatic triglycerides, IL6, IL10, caspase 3 and AMPK (adenosine monophosphate‐activated protein kinase). A signifi...
Source: Physiological Reports - Category: Physiology Authors: Tags: Original Research Source Type: research