Synthesis and characterization of chemically-reactive solubilization surfactants for membrane proteins and preparation of membrane protein hydrogel microfibers

Publication date: September 2019Source: Colloid and Interface Science Communications, Volume 32Author(s): Aki Taniguchi, Shuhei Koeda, Tomoyasu Noji, Keisuke Kawakami, Natsumi Sumito, Takehisa Dewa, Shigeru Itoh, Nobuo Kamiya, Toshihisa MizunoAbstractWe herein report the synthesis and characterization of unique chemically reactive solubilization surfactants, Alk-DKDKC12K and Bis-alk-DKDKC12K that consist of the DKDKC12K unit, previously reported to function as a novel class of lipopeptide-based solubilization surfactant for membrane proteins such as photosystem I (PSI) derived from cyanobacteria, and one or two alkyne groups at the N- and/or C-terminal sides, which could react with bis-azide crosslinker via Hüisgen cycloaddition reaction. By a stepwise in situ crosslinking of PSI solubilized in a buffer, containing Alk-DKDKC12K and Bis-alk-DKDKC12K, with a set of crosslinkers (the first one is bis azide compounds and the second is formylated dextran), we succeeded in preparing hydrogel microfibers encapsulating PSI without damage to the original photophysical functions.Graphical abstract
Source: Colloids and Interface Science Communications - Category: Nanotechnology Source Type: research