A novel antioxidant rich compound 2-hydoxy 4-methylbenzaldehyde from Decalepis arayalpathra induces apoptosis in breast cancer cells

In this study, we reported the biomedical potentials of 2-hydoxy 4-methylbenzaldehyde (2H4MB) from D. arayalpathra. The compound 2H4MB was isolated from fleshy root barks of the plants using steam-hydro distillation extraction method and quantified using HPLC analysis, which yielded 6.5 ± 0.8 μg g−1 of purified compound. Further, the antioxidant and anticancer effects in vitro were studied in detail using MDA-MB-231 cells by cell-based assays. 2H4MB was observed to display the features such as generation of reactive oxygen species (ROS), improved cytotoxicity, loss of mitochondrial membrane (Δψm) potential, and nuclear DNA fragmentations, leading to induce cellular apoptosis and cell cycle arrest. Further, the bioavailability of 2H4MB may facilitate its use as a potential cancer therapeutic agent.Graphical abstract
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research