Epidemiological distribution of genotypes of Giardia duodenalis in humans in Spain

Although the distribution of Giardia duodenalis genotypes in humans has been increasingly reported in recent years, data on possible differences in pathogen transmission between age groups and virulence between g...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research

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Giardiasis is a common diarrhoeal disease caused by the protozoan Giardia duodenalis. It is prevalent in low-income countries in the context of inadequate access to water, sanitation and hygiene (WASH), and is fr...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
Giardia lamblia is usually cultured axenically in TYI-S-33, a complex medium which does not permit survival and growth of mammalian cells. Likewise, medium commonly used to maintain and grow mammalian cells does not support healthy trophozoite survival for more than a few hours. The inability to coculture trophozoites and epithelial cells under optimal conditions limits studies of their interactions as well as interpretation of results. Trophozoites of the WB isolate but not the GS isolate were repeatedly adapted to grow stably in long-term cocultures with Caco2, Cos7, and mouse tumor rectal (RIT) cell lines using hybridom...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Source Type: research
Publication date: Available online 15 October 2019Source: TrAC Trends in Analytical ChemistryAuthor(s): Sona Jain, Thyago Gois Costa Melo, Silvio Santana Dolabella, Juewen LiuAbstractWater-borne protozoan parasites like Cryptosporidium, Giardia, Entamoeba and Toxoplasma are responsible for 1.7 billion cases of diarrhea globally, representing the second most frequent reason for mortality among children under five. They show an environmentally resistant cyst/oocyst stage. Despite their importance, the standard analytical methods for their detection still rely on immunostaining followed by microscopic analysis. In recent year...
Source: TrAC Trends in Analytical Chemistry - Category: Chemistry Source Type: research
Abstract Phosphoinositides (or phosphatidylinositol phosphates, PIPs) are low-abundance membrane phospholipids that act, in conjunction with their binding partners, as important constitutive signals defining biochemical organelle identity as well as membrane trafficking and signal transduction at eukaryotic cellular membranes. In this review, we present roles for PIP residues and PIP-binding proteins in endocytosis and autophagy in protist parasites such as Trypanosoma brucei, Toxoplasma gondii, Plasmodium falciparum, Entamoeba histolytica, and Giardia lamblia. Molecular parasitologists with an interest in compara...
Source: Trends in Parasitology - Category: Parasitology Authors: Tags: Trends Parasitol Source Type: research
AbstractGiardia intestinalis andBlastocystis spp. are two common zoonotic intestinal parasites responsible for the majority of diarrheic cases, especially in children. The study was planned to determine the impact role of bothGiardia andBlastocystis co-infection in children up to 12  years old with acute diarrhea in Beni-Suef, Egypt. Also, to estimate the general prevalence rates and risk factors associated with intestinal parasitic infections. Stool samples collected from 125 children, within a period of 7 months, were examined using direct wet mount and trichrome stain in c omparison to in vitro culture on Jone...
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research
Publication date: 5 January 2020Source: Journal of Molecular Structure, Volume 1199Author(s): Gurjaspreet Singh, Pinky Satija, Baljinder Singh, Shweta Sinha, Rakesh Sehgal, Subash Chandra SahooAbstractThis work explores the facile synthesis of a series of antipyrine acetylinic framework (3a-3h). The newly synthesized compounds are recognized by IR, NMR (1H, 13C), X-ray diffraction. This collection played indispensable role in the development of biologically active agents. Our goal is to enable adoption of green chemistry within the pharmaceutical industry for the benefit of society, industry and the environment. This work ...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
We describe the follow-up of a patient with recurrent giardiasis refractory to nitroimidazoles. Despite the different therapies received, the symptomatology and parasitic forms of G. lamblia persisted in the patient. There is no standard treatment regimen for giardiasis refractory to nitroimidazoles. When treatment failure is confirmed, it is necessary to switch to second-line regimens. PMID: 31545780 [PubMed - in process]
Source: Infezioni in Medicina - Category: Infectious Diseases Tags: Infez Med Source Type: research
Conclusion: High rate of intestinal parasites particularly, S. stercoralis compared to the most recent studies of general population. Therefore, regular screening and tracking the positive cases, disinfection of the living environment, training and financing of the staff, increasing the number of the workers, recruiting of professionals and trained personnel in these centers are suggested. PMID: 31543921 [PubMed]
Source: Iranian Journal of Parasitology - Category: Parasitology Tags: Iran J Parasitol Source Type: research
N, Pumarola T, Espasa M, Sulleiro E Abstract Sexually transmitted infections (STIs) are a major problem worldwide. In addition, the spectrum of STIs is now expanding, including parasitic, bacterial, and viral infections. The study retrospectively describes the presence of enteric pathogens among 73 patients with gastrointestinal symptoms of enteritis and proctocolitis attending to an STI unit in Barcelona, Spain, between 2015 and 2016. Only patients investigated for intestinal parasitic infections were included in the study. Different diagnostic procedures were established for the detection of parasites, bacterial...
Source: The American Journal of Tropical Medicine and Hygiene - Category: Tropical Medicine Authors: Tags: Am J Trop Med Hyg Source Type: research
, Quílez J Abstract Giardia duodenalis is one of the most prevalent human intestinal parasite, with children living in developing countries being particularly at risk of infection. The occurrence and molecular diversity of G. duodenalis was investigated in stools specimens from 307 individuals aged one to nineteen years in Colombia. Samples were collected in three educational establishments (n: 163) and two hospital laboratories (n: 144) from urban and rural areas. Feces were concentrated using a biphasic sedimentation method and wet mounts of the sediment were examined by light microscopy. G. duodenalis as...
Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Tags: Infect Genet Evol Source Type: research
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