Aptamer-Based Affinity Chromatography for Protein Extraction and Purification.

Aptamer-Based Affinity Chromatography for Protein Extraction and Purification. Adv Biochem Eng Biotechnol. 2019 Sep 05;: Authors: Perret G, Boschetti E Abstract Aptamers are oligonucleotide molecules able to recognize very specifically proteins. Among the possible applications, aptamers have been used for affinity chromatography with effective results and advantages over most advanced protein separation technologies. This chapter first discusses the context of the affinity chromatography with aptamer ligands. With the adaptation of SELEX, the chemical modifications of aptamers to comply with the covalent coupling and the separation process are then extensively presented. A focus is then made about the most important applications for protein separation with real-life examples and the comparison with immunoaffinity chromatography. In spite of well-advanced demonstrations and the extraordinary potential developments, a significant optimization work is still due to deserve large-scale applications with all necessary validations. Graphical Abstract Aptamer-protein complexes by X-ray crystallography. PMID: 31485702 [PubMed - as supplied by publisher]
Source: Advances in Biochemical Engineering Biotechnology - Category: Biotechnology Authors: Tags: Adv Biochem Eng Biotechnol Source Type: research