Myristica fragrans bio-active ester functionalized ZnO nanoparticles exhibit antibacterial and antibiofilm activities in clinical isolates

Publication date: Available online 6 September 2019Source: Journal of Microbiological MethodsAuthor(s): Tijo Cherian, Khursheed Ali, Saher Fatima, Quaiser Saquib, Sabiha M. Ansari, Hend A. Alwathnani, Abdulaziz A. Al-Khedhairy, Majed Al-Shaeri, Javed MusarratAbstractWe provide a novel one-step/one-pot bio-inspired method of synthesis for Myristica fragrans leaf ester (MFLE) capped‑zinc oxide nanoparticles (MFLE-ZnONPs). Antibacterial and antbiofilm efficacies of MFLE-ZnONPs were tested against the multi-drug resistant (MDR) Escherichia coli (E. coli-336), methicillin-resistant Staphylococcus aureus (MRSA-1) and methicillin-sensitive (MSSA-2) clinical isolates. Antibacterial screening using well diffusion assay revealed the cytotoxicity of MFLE-ZnONPs in the range of 500-2000 μg/ml. MFLE-ZnONPs significantly increased the zone of growth inhibition of E. coli-336 (17.0 ± 0.5 to 19.25 ± 1.0 mm), MSSA-2 (16.75 ± 0.8 to 19.0 ± 0.7 mm) and MRSA-1 (16.25 ± 1.0 to 18.25 ± 0.5 mm), respectively. The minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC) against E. coli-336, MRSA-1 and MSSA-2 were found to be 1500, 1000 and 500 μg/ml, and 2500, 2000 and 1500 μg/ml, respectively. A time and dose dependent reduction in the cell proliferation were also found at the respective MICs of tested strains. Scanning electron microscopy (SEM) of MFLE-ZnONPs-treated strains exhibited cellular damage via loss of native rod ...
Source: Journal of Microbiological Methods - Category: Microbiology Source Type: research