Correlation among automated scores of agglutination, antigen density by flow cytometry and genetics of D variants

Laboratory testing to identify the molecular basis of serologic weak D phenotypes is recommended to determine whether a pregnant woman or potential transfusion recipient should be managed as RhD-positive or RhD-negative. The variation in D antigen expression on RBCs, different potencies of anti-D typing reagents, lack of standardized test methods, and the subjectivity of interpreting agglutination reactions complicate the detection of D variants. We evaluated the correlation of agglutination scores by an automated immunoassay analyzer with D antigen densities determined by flow cytometry, and D variant types identified by molecular analysis.

Source: Transfusion and Apheresis Science - September 4, 2019 Category: Hematology Authors: Carine Prisco Arnoni, Jana ína Guilhem Muniz, Tatiane Aparecida de Paula Vendrame, Rosangela de Medeiros, Afonso José Pereira Cortez, Flavia Roche Moreira Latini, Lilian Castilho Source Type: research