Pyridoxine and pancreatic acinar cells: transport physiology and effect on gene expression profile.

Pyridoxine and pancreatic acinar cells: transport physiology and effect on gene expression profile. Am J Physiol Cell Physiol. 2019 Sep 04;: Authors: Srinivasan P, Ramesh V, Wu J, Heskett CW, Chu BD, Said HM Abstract Pyridoxine (vitamin B6), an essential micronutrient for normal cell physiology, plays an important role in the function of the exocrine pancreas. Pancreatic acinar cells (PACs) obtain vitamin B6 from circulation, but little is known about the mechanism involved in the uptake process; limited information also exists on the effect of pyridoxine availability on the gene expression profile in these cells. We addressed both these issues in the current investigation using mouse-derived pancreatic acinar 266-6 cells (PAC 266-6) and human primary PACs (hPACs; obtained from organ donors), together with appropriate physiological and molecular (RNA-Seq) approaches. The results showed that [3H]-pyridoxine uptake to be: 1) pH- and temperature- (but not Na) dependent; 2) saturable as a function of concentration; 3) cis-inhibited by unlabeled pyridoxine and its close structural analogs; 4) trans-stimulated by unlabeled pyridoxine; 5) regulated by an intracellular Ca2+/calmodulin-mediated pathway; 6) adaptively-regulated by extracellular substrate (pyridoxine) availability; 7) negatively impacted by exposure to cigarette smoke extract. Vitamin B6 availability was found (by means of RNA-Seq) to significantly (FDR < 0.05) modulate the ...
Source: Am J Physiol Cell Ph... - Category: Cytology Authors: Tags: Am J Physiol Cell Physiol Source Type: research