Photoclick chemistry to create dextran-based nucleic acid microarrays.

Photoclick chemistry to create dextran-based nucleic acid microarrays.
Anal Bioanal Chem. 2019 Sep 03;:
Authors: Díaz-Betancor Z, Bañuls MJ, Maquieira Á
Abstract
In the literature, there are reports of the utilization of various hydrogels to create generic platforms for protein microarray applications. Here, a novel strategy was developed to obtain high-performance microarrays. In it, a dextran hydrogel is used to covalently immobilize oligonucleotides and proteins. This method employs aqueous solutions of dextran methacrylate (Dx-MA), which is a biocompatible photopolymerizable monomer. Capture probes are immobilized inside the hydrogel via a light-induced thiol-acrylate coupling reaction at the same time as the dextran polymer is formed. Hydrogel microarrays based on this technique were prepared on different surfaces, such as a Blu-ray Disk and polycarbonate or alkene-functionalized glass slides, and these systems showed high probe-loading capabilities and good biorecognition yields. This methodology presents advantages such as a low cost, a short analysis time, a low limit of detection, and multiplexing capabilities, among others. Confocal fluorescence microscopy analysis demonstrated that in these hydrogel-based microarrays, receptor immobilization and the biorecognition event occurred within the hydrogel and not merely on the surface.
PMID: 31482291 [PubMed - as supplied by publisher]

Source: Analytical and Bioanalytical Chemistry - September 2, 2019 Category: Chemistry Authors: Díaz-Betancor Z, Bañuls MJ, Maquieira Á Tags: Anal Bioanal Chem Source Type: research