Cancers, Vol. 11, Pages 1308: GLUT1 and TUBB4 in Glioblastoma Could be Efficacious Targets

Cancers, Vol. 11, Pages 1308: GLUT1 and TUBB4 in Glioblastoma Could be Efficacious Targets Cancers doi: 10.3390/cancers11091308 Authors: Maheedhara R. Guda Collin M. Labak Sara Ibrahim Omar Swapna Asuthkar Subra Airala Jack Tuszynski Andrew J. Tsung Kiran K. Velpula Glioblastoma multiforme (GBM) is the most aggressive and deadly brain tumor, portending a median 13-month survival even following gross total resection with adjuvant chemotherapy and radiotherapy. This prognosis necessitates improved therapies for the disease. A target of interest for novel chemotherapies is the Warburg Effect, which describes the tumor’s shift away from oxidative phosphorylation towards glycolysis. Here, we elucidate GLUT1 (Glucose transporter 1) and one of its associated binding partners, TUBB4 (Tubulin 4), as potentially druggable targets in GBM. Using data mining approach, we demonstrate that GLUT1 is overexpressed as a function of tumor grade in astrocytoma’s and that its overexpression is associated with poorer prognosis. Using both mass spectrometry performed on hGBM (human glioblastoma patient specimen) and in silico modeling, we show that GLUT1 interacts with TUBB4, and more accurately demonstrates GLUT1’s binding with fasentin. Proximity ligation assay (PLA) and immunoprecipitation studies confirm GLUT1 interaction with TUBB4. Treatment of GSC33 and GSC28 cells with TUBB4 inhibitor, CR-42-24, reduces the expression of GLUT1 however, TUB...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research

Related Links:

Purpose of review The current review summarizes recent advances on three important issues in neurofibromatosis type 1 (NF1) management: the identification of specific NF1 gene mutations predicting the risk for developing neurological malignancies; the molecular features of NF1-associated tumors and their differences from sporadic neoplasms; genetic, epigenetic, or microenviromental factors leading benign tumors to a malignant transformation in NF1. Recent findings The association between the risk of developing optic pathway glioma and specific germiline NF1 mutations is still debated and further studies are needed wit...
Source: Current Opinion in Oncology - Category: Cancer & Oncology Tags: BRAIN AND NERVOUS SYSTEM: Edited by Marc Sanson Source Type: research
Authors: Steponaitis G, Kazlauskas A, Vaitkienė P, Deltuva VP, Mikuciunas M, Skiriutė D Abstract Background: Gliomas are the most common and aggressive among primary malignant brain tumours with significant inter- and intratumour heterogeneity in histology, molecular profile, and patient outcome. However, molecular targets that could provide reliable diagnostic and prognostic information on this type of cancer are currently unknown. Recent studies show that certain phenotypes of gliomas such as malignancy, resistance to therapy, and relapses are associated with the epigenetic alterations of tumour-specific genes....
Source: Journal of Oncology - Category: Cancer & Oncology Tags: J Oncol Source Type: research
Publication date: 20 August 2019Source: Cell Reports, Volume 28, Issue 8Author(s): Hanna Sabelström, Rebecca Petri, Ksenya Shchors, Rahul Jandial, Christin Schmidt, Rohit Sacheva, Selma Masic, Edith Yuan, Trenten Fenster, Michael Martinez, Supna Saxena, Theodore P. Nicolaides, Shirin Ilkhanizadeh, Mitchel S. Berger, Evan Y. Snyder, William A. Weiss, Johan Jakobsson, Anders I. PerssonSummaryIdentifying cellular programs that drive cancers to be stem-like and treatment resistant is critical to improving outcomes in patients. Here, we demonstrate that constitutive extracellular signal-regulated kinase 1/2 (ERK1/2) activa...
Source: Cell Reports - Category: Cytology Source Type: research
AbstractThe brain tumours represent a complex tissue that has its own characteristic metabolic features and is interfaced with the whole organism. We investigated changes in basal blood plasma metabolites in the presence of primary brain tumour, their correlation with tumour grade, as well as the feasibility of statistical discrimination based on plasma metabolites. Together 60 plasma samples from patients with clinically defined glioblastoma, meningioma, oligodendrioglioma, astrocytoma, and non ‐specific glial tumour and plasma samples from 28 healthy volunteers without any cancer history were measured by NMR spectrosco...
Source: IUBMB Life - Category: Research Authors: Tags: RESEARCH COMMUNICATION Source Type: research
Conclusions: Our analysis did not yield evidence of strong effects of rural compared with urban residence or ses strata on survival in brain cancer. However, some variation in survival for patients with specific histologies warrants further research into the mechanisms by which rural or urban residence and income stratum influences survival. PMID: 31285671 [PubMed - in process]
Source: Current Oncology - Category: Cancer & Oncology Tags: Curr Oncol Source Type: research
Background: Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor which levels was shown to have a tight coherence with various types of cancer. AREG was also designated to be a promising marker for several types of cancer however precious little data about AREG role in the most frequent and generally lethal human brain tumours - astrocytomas reported up to date. The aim of the study was to investigate how AREG changes at epigenetic and expression levels reflect on astrocytoma malignancy and patient outcome.Methods: In total 205 low and high grade astrocytoma samples (15 pilocytic astrocytomas, ...
Source: Journal of Cancer - Category: Cancer & Oncology Authors: Tags: Research Paper Source Type: research
Conditions:   High Grade Glioma;   Anaplastic Astrocytoma;   Anaplastic Oligodendroglioma;   Glioblastoma Interventions:   Drug: Regadenoson 0.05mg;   Drug: Regadensoson 0.1mg;   Drug: Regadensoson 0.2mg;   Drug: Regadensoson 0.4mg;   Drug: Regadensoson 0.7mg;   Drug: Regadensoson 1.0mg;   Drug: Regadensoson 1.4mg Sponsors:   Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins;   National Cancer Institute (NCI) Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Conclusions: 18F-Fluoroglutamine PET appears sensitive for diagnosis of early/late progression of brain tumor progression post-treatment.
Source: Journal of Nuclear Medicine - Category: Nuclear Medicine Authors: Tags: Novel Brain Imaging Targets Source Type: research
Conclusions: The prediction of the WHO 2016 classification of newly-diagnosed gliomas by 18F-FDopa PET is significantly enhanced when combining the analyses of dynamic with static PET parameters and especially, time-to-peak with the metabolic tumor volume. Figure 1. Distribution of median values (points) and interquartile ranges (colored rectangles) according to both metabolic tumor volume (MTV) and time-to-peak (TTP) expressed with natural logarithm, for IDH-wildtype (blue) and IDH-mutant (grey) astrocytomas, IDH-mutant and 1p/19q co-deleted oligodendrogliomas (red) and IDH-wildtype-glioblastomas (green). The single case ...
Source: Journal of Nuclear Medicine - Category: Nuclear Medicine Authors: Tags: Head and Neck Cancer Source Type: research
Antonio Lucena-Cacace1, Masayuki Umeda1, Lola E. Navas2,3 and Amancio Carnero2,3* 1Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan 2CIBERONC, ISCIII, Madrid, Spain 3Instituto de Biomedicina de Sevilla (IBIS), Hospital Universitario Virgen del Rocío (HUVR), CSIC, Universidad de Sevilla, Sevilla, Spain Glioma Cancer Stem-Like Cells (GSCs) are a small subset of CD133+ cells with self-renewal properties and capable of initiating new tumors contributing to Glioma progression, maintenance, hierarchy, and complexity. GSCs are highly res...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
More News: Astrocytoma | Brain | Brain Cancers | Brain Tumor | Cancer | Cancer & Oncology | Chemotherapy | Nanotechnology | Neurology | Stem Cell Therapy | Stem Cells | Study