Distribution of regulatory T cells in inflammatory colorectal polyps of Miniature Dachshunds

Publication date: Available online 3 September 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Keisuke Konishi, Hirotaka Igarashi, Shingo Maeda, Eri Uchida, Kiwamu Hanazono, Takashi Tamamoto, Kazuyuki Uchida, Daiji Endoh, Koichi OhnoAbstractInflammatory colorectal polyp (ICRP) is an emerging disease in Miniature Dachshunds (MDs). Animals with this disease exhibit multiple polyps with severe neutrophil infiltration that respond to immunosuppressive therapy. Macrophages in polypoid lesions have been described to play an important role in neutrophil infiltration in the lesion by producing IL-8. In contrast, IL-10, an anti-inflammatory cytokine, was also reported to be upregulated in polypoid lesions, but its significance in the pathogenesis of ICRP has not been clarified. Regulatory T cells (Tregs) are the main source of IL-10 production and contribute to the maintenance of intestinal homeostasis. Therefore, the objective of this research was to compare the distribution of Tregs in polypoid lesions of ICRPs and the association between the distribution and expression of pro- or anti-inflammatory cytokines. Tissue biopsy specimens of polypoid lesions were collected from 28 MDs with ICRP. Those of macroscopically non-polypoid colonic mucosa from 24 MDs with ICRPs and 21 control dogs were further included as controls. Real-time quantitative polymerase chain reaction was used to quantify gene expression of IL-1β, IL-4, IL-6, IL-8, IL-10, IL-17, IL-22, IFN-γ, TNF-α,...
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research