Therapeutic Effect of Deferrioxamine Conjugated to PEGylated Gold Nanoparticles and Complexed with Mn(II) Beside the CT Scan and MRI Diagnostic Studies

Publication date: Available online 3 September 2019Source: Colloids and Surfaces A: Physicochemical and Engineering AspectsAuthor(s): Fatemeh Yaghoobi, Reza Karimi Shervedani, Mostafa Torabi, Amirhosein Kefayat, Fatemeh Ghahremani, Amin FarzadniyaAbstractSynthesis, characterization, and in-vitro/in-vivo examinations of a theranostic system with novel formulation and straightforward synthesis method are reported in this work. Deferrioxamine-manganese(II), as a theranostic complex (Deferrioxamine as therapeutic and manganese(II) as MRI imaging agent) is conjugated in adjacent to folic acid with the PEGylated gold nanoparticles, leading to formation of biodegradable and biocompatible AuNPs-PEG-[DFO-Mn(II)]‖-[FOA] system. The fabrication process is followed by several characterization methods including TEM, DLS, ζ-potential, FTIR, XPS, UV-Vis, and fluorescence.Initially, the characterized system was tested as a contrast agent by MRI and X-ray CT-scan techniques. The values obtained for relaxation rate and the slope of Hounsfield unit vs. concentration, 12.0 mM―1 s―1 and 8.27 HU L/g, supported efficiency of the system for dual imaging applications. In addition, an obvious T1-weighed contrast was observed by in-vivo method. The intracellular delivery of the system was monitored by flow cytometry and florescence microscopy imaging based on PI florescent dye attached to the AuNPs as AuNPs-PEG-[PI]‖-[FOA].Then, anticancer activity of the system was evaluated by in-vivo expe...
Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Category: Chemistry Source Type: research