600 Differential Molecular Signature of Dermal Papilla Cells (DPCs) from Balding and Non balding Scalp in 2D, 3D culture and Co-culture with Adipose-derived stem cells (ASCs)

Androgenic alopecia affects hair follicles (HFs) from frontal and vertex area. Hair inductive properties of DPCs are lost in bi-dimentional (2D) culture. In a recent study three dimentional (3D) structure of cultured DPCs was able to recapitulate 22% of molecular signature of the intact dermal papilla. The aim of this study is to compare gene expression differences between DPCs from balding scalp and non balding scalp (occipital area) and their changes in 2D and 3D cultures. We investigated whether 3D structure of these immortalized cells and their co-culture with ASCs could increase the expression of hair inductivity markers.
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Tags: Stem Cells, Skin Appendages and Tissue Regeneration Source Type: research