Regenerative effects of glycyrrhizin and/or platelet rich plasma on type II collagen induced arthritis: Targeting autophay machinery markers, inflammation and oxidative stress.

Regenerative effects of glycyrrhizin and/or platelet rich plasma on type II collagen induced arthritis: Targeting autophay machinery markers, inflammation and oxidative stress. Arch Biochem Biophys. 2019 Aug 30;:108095 Authors: Shafik NM, El-Deeb OS, El-Esawy RO, Mohamed DA, Deghidy EA Abstract Rheumatoid arthritis (RA) is a systemic chronic autoimmune disease manifested by joint destruction and deformity, hence decreasing life's quality. The aim of the present work is to explore the mechanistic effects of platelet rich plasma (PRP) and/or glycyrrhizin (GL) treatments on collagen induced arthritis. 75 female Wistar rats were allocated into five equal groups. Group I: control group. Group II: arthritis group (A group); arthritis was induced by collagen type-II. Group III: Glycyrrhizin (A+GL) group, Group IV: platelet rich plasma treated (A+PRP) group and Group V: combined treatment (A+GL+PRP) group. Hind paw joint tissue levels of high-mobility group box 1protein (HMGB-1), beclin-1 and nuclear factor (erythroid-2)-related factor 2 (Nrf2) DNA binding activity were detected by ELISA. Activities of myeloperoxidase (MPO) and catalase enzymes were determined spectrophotometrically. mRNA expression levels of microtubule associated protein light chain 3 (LC3) was detected by quantitative real time PCR. After 8 weeks treatment, there was improvement of inflammation and autophagy biomarkers by the significant reduction of HMGB-1and beclin-1 le...
Source: Archives of Biochemistry and Biophysics - Category: Biochemistry Authors: Tags: Arch Biochem Biophys Source Type: research