Comparison of Reverse Transcription Loop-Mediated Isothermal Amplification Method with SYBR Green Real-Time RT-PCR and Direct Fluorescent Antibody Test for diagnosis of rabies.
This study tried to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of rabies virus RNA in the brain samples in comparison with SYBR Green Real Time RT-PCR test as a molecular technique and Direct Fluorescent Antibody Test as a serologic method. In this study, RT-LAMP was developed to rabies diagnosis. Six primers were designed, based on the nucleoprotein (N) of rabies virus. The sensitivity and specificity of SYBR Green Real-Time RT-PCR and RT-LAMP methods were also determined.RT-LAMP optimized at 58˚C for 60 min and the sensitivity and specificity of RT-LAMP and SYBR Green Real-Time RT-PCR were 91.2%, 84.2%, 94.12% and 88.9%, respectively. The slight difference between the sensitivity and specificity of RT-LAMP and SYBR Green Real-Time RT-PCR demonstrated that RT-LAMP can be used as a reliable and cost-effective method for diagnosis of rabies.
PMID: 31474697 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Naji E, Fadajan Z, Afshar D, Fazeli M Tags: Jpn J Infect Dis Source Type: research
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