Comparison of Reverse Transcription Loop-Mediated Isothermal Amplification Method with SYBR Green Real-Time RT-PCR and Direct Fluorescent Antibody Test for diagnosis of rabies.

This study tried to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of rabies virus RNA in the brain samples in comparison with SYBR Green Real Time RT-PCR test as a molecular technique and Direct Fluorescent Antibody Test as a serologic method. In this study, RT-LAMP was developed to rabies diagnosis. Six primers were designed, based on the nucleoprotein (N) of rabies virus. The sensitivity and specificity of SYBR Green Real-Time RT-PCR and RT-LAMP methods were also determined.RT-LAMP optimized at 58˚C for 60 min and the sensitivity and specificity of RT-LAMP and SYBR Green Real-Time RT-PCR were 91.2%, 84.2%, 94.12% and 88.9%, respectively. The slight difference between the sensitivity and specificity of RT-LAMP and SYBR Green Real-Time RT-PCR demonstrated that RT-LAMP can be used as a reliable and cost-effective method for diagnosis of rabies. PMID: 31474697 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research

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This article provides an update on changes in case definitions that have been made since the case definitions were last published in 2009, and describes updates that have been made to the interactive website since 2013. Changes were made to the case definitions of five diseases. For hepatitis C, the new case definition now distinguishes between acute and chronic infection. For cyclosporiasis, the probable case definition requires an epidemiologic link, with the clarification that this would likely be due to exposure to a common food source. For rabies, the probable case definition now refers to detection of rabies-neutrali...
Source: Can Commun Dis Rep - Category: Infectious Diseases Authors: Tags: Can Commun Dis Rep Source Type: research
Conclusions: Health-seeking behavior and compliance to complete course of anti-rabies vaccination is unsatisfactory, which has to be improved to prevent rabies.
Source: Indian Journal of Public Health - Category: International Medicine & Public Health Authors: Source Type: research
by Victoria J. Brookes, Salome D ürr, Michael P. Ward Canine rabies was endemic pre-urbanisation, yet little is known about how it persists in small populations of dogs typically seen in rural and remote regions. By simulating rabies outbreaks in such populations (50–90 dogs) using a network-based model, our objective was to determine if rabies-ind uced behavioural changes influence disease persistence. Behavioural changes–increased bite frequency and increased number or duration of contacts (disease-induced roaming or paralysis, respectively)–were found to be essential for disease propagation. Spre...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
CONCLUSION: This study concludes that respondents have relatively limited knowledge, inconsistent attitude and poor practices towards Rabies prevention and control. There is a high need of imparting knowledge to the population from multidisciplinary programs for effective management and prevention of Rabies. PMID: 31491398 [PubMed - as supplied by publisher]
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research
Canine rabies is endemic in Haiti and the Dominican Republic.
Source: CDC Morbidity and Mortality Weekly Report - Category: American Health Tags: Animal-Related Diseases Dog Bites Eye and Vision Health MMWR Morbidity & Mortality Weekly Report Rabies Source Type: news
by Ricardo Castillo-Neyra, Amparo M. Toledo, Claudia Arevalo-Nieto, Hannelore MacDonald, Micaela De la Puente-Le ón, Cesar Naquira-Velarde, Valerie A. Paz-Soldan, Alison M. Buttenheim, Michael Z. Levy To control and prevent rabies in Latin America, mass dog vaccination campaigns (MDVC) are implemented mainly through fixed-location vaccination points: owners have to bring their dogs to the vaccination points where they receive the vaccination free of charge. Dog rabies is still endemic in some L atin-American countries and high overall dog vaccination coverage and even distribution of vaccinated dogs are desired att...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Discussion Dengue is an important arboviral infection that affects about 40% of the world population. It is found mainly in topical and subtropical areas of the world mainly in developing countries but it range is spreading including the United States. A review of common arboviruses can be found here. It is a flaviavirus with 4 distinct serotypes named DENV-1 through DENV-4 and is spread by A. aegypti a day biting mosquito. Infection with one serotype confers immunity to that serotype but not the others. It does offer some protection for cross-infection but this only lasts a few months. Incubation period is 3-14 days with ...
Source: PediatricEducation.org - Category: Pediatrics Authors: Tags: Uncategorized Source Type: news
This study evaluated the utility of a lateral flow immunochromatographic assay (LFA) (Anigen Rapid Rabies Ag Test Kit, Bionote, Hwaseong-si, Korea) for rapid post mortem diagnosis of rabies in dogs. Brain tissue was collected from 202 animals that were screened through the Government of Goa rabies surveillance system. The brain tissue samples were obtained from 188 dogs, nine cats, three bovines, one jackal and one monkey. In addition, 10 dogs that died due to trauma from road accidents were included as negative controls for the study. The diagnostic performance of LFA was evaluated using results from direct fluorescence a...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
In this study, no strains circulating in Zimbabwe were detected in Zambia.
Source: Virus Genes - Category: Genetics & Stem Cells Source Type: research
CONCLUSIONS.: The groupings of viral sequences show that the cases of canine rabies reported in Arequipa are the result of the expansion of canine rabies from the endemic region of Puno. PMID: 31116337 [PubMed - in process]
Source: Revista Peruana de Medicina de Experimental y Salud Publica - Category: International Medicine & Public Health Tags: Rev Peru Med Exp Salud Publica Source Type: research
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