Prevalence of Virulence Genes of Diarrheagenic Escherichia coli in Fecal Samples Obtained from Cattle, Poultry and Diarrheic Patients in Bangladesh.

Prevalence of Virulence Genes of Diarrheagenic Escherichia coli in Fecal Samples Obtained from Cattle, Poultry and Diarrheic Patients in Bangladesh. Jpn J Infect Dis. 2019 Aug 30;: Authors: Parvej MS, Alam MA, Shono M, Zahan MN, Masuma Parvez MM, Karim Ansari W, Jowel MS, Uddin MS, Kage-Nakadai E, Rahman MT, Nishikawa Y Abstract Using multiplex real-time PCR, 960 fecal samples collected from poultry, cattle, and diarrheic patients in Bangladesh were screened for diarrheagenic Escherichia coli (DEC). Invasion-related gene virB had the highest prevalence in human patients (41%) and was shown to be positively correlated first with afaB for diffuse adhesion and second with aggR for aggregative adhesion. These three genes were specific to patients. In contrast, Shiga toxin genes stx1 (57%) and stx2 (40%) were prevalent in cattle samples. eae for attaching and effacing lesion, and elt and est of enterotoxins were detected from all three sample sources. Heat map and hierarchical clustering assigned the samples into five different clusters with patient samples positive for virB and afaB placing together in one cluster. Although detection of virulence genes cannot be a direct indication of the distribution of DEC organisms, their detection suggests that Shigella spp. or enteroinvasive E. coli are the most prevalent among diarrheagenic bacteria in Bangladesh and that diffusely adherent E. coli are concomitant bacterium. eae-possessing organisms in patients may com...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research

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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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Source: The Lancet Global Health - Category: International Medicine & Public Health Source Type: research
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Source: The Lancet Global Health - Category: International Medicine & Public Health Source Type: research
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Source: Microbiological Research - Category: Infectious Diseases Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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Source: Journal of Animal Physiology and Animal Nutrition - Category: Zoology Authors: Tags: ORIGINAL ARTICLE Source Type: research
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Source: Gut Pathogens - Category: Microbiology Source Type: research
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Source: Current Topics in Microbiology and Immunology - Category: Microbiology Tags: Curr Top Microbiol Immunol Source Type: research
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