Intervention of bio-protective endophyte Bacillus tequilensis enhance physiological strength of tomato during Fusarium wilt infection

Publication date: Available online 2 September 2019Source: Biological ControlAuthor(s): Arpita Bhattacharya, Ved Prakash Giri, Satyendra Pratap Singh, Shipra Pandey, Priyanka Chauhan, Sumit Kumar Soni, Suchi Srivastava, Poonam C. Singh, Aradhana MishraAbstractThe plants in their natural environment face various biotic stresses due to invading phytopathogens. Their control measures primarily involve large scale applications of chemical fungicides. The excessive use of chemicals leads to the contamination of the environment by entering the food web. To address the above concern, in the present study six antagonistic bacterial strains were screened from 344 isolates and identified through 16S rRNA sequencing. Out of these six antagonistic bacterial endophytes, Bacillus tequilensis (PBE1) (MTCC25188) was proven most effective and an eco-friendly substitute of chemical fungicides against Fusarium oxysporum for tomato wilt disease management. The antagonistic effect of PBE1 was well corroborated by dual culture plate assay and SEM micrographs. The PBE1 also showed efficient plant growth-promoting traits as it produced indole acetic acid (IAA) (31.89 μg ml−1), hydroxymate type siderophore (5.00 μg ml−1) along with phosphate solubilizing (9.09 μg ml−1) ability. The bio-protective intervention of PBE1 demonstrated 60.00% reduction of disease incidence in comparison to the pathogen infected plants. Simultaneously, enhanced physical parameters such as root length, shoot length,...
Source: Biological Control - Category: Biology Source Type: research