New photothermal immunoassay of human chorionic gonadotropin using Prussian blue nanoparticle-based photothermal conversion.
New photothermal immunoassay of human chorionic gonadotropin using Prussian blue nanoparticle-based photothermal conversion.
Anal Bioanal Chem. 2019 Aug 30;:
Authors: Hong G, Zhang D, He Y, Yang Y, Chen P, Yang H, Zhou Z, Liu Y, Wang Y
Abstract
A new near-infrared-based photothermal immunosensing strategy was developed for the sensitive and feasible detection of human chorionic gonadotropin (HCG) by use of a Prussian blue nanoparticle-based photothermal conversion system. Prussian blue nanospheres synthesized by the one-pot method were used for the labeling of anti-HCG detection antibody. A sandwich-type immunoreaction was initially conducted on a monoclonal anti-HCG antibody-coated microplate with a nanoparticle-labeled signal antibody. Accompanying formation of the sandwiched immunocomplex, Prussian blue nanospheres caused photothermal conversion under 980-nm laser irradiation, thereby resulting in an increase of the temperature of the detection system measured by a portable digital thermometer. The properties and factors influencing the analytical performance of the photothermal immunoassay were studied in detail. Under the optimal conditions, the Prussian blue nanoparticle-based photothermal immunoassay exhibited good temperature responses relative to target HCG concentrations within the dynamic range of 0.01-100 ng mL-1 at a low detection limit of 5.8 pg mL-1. This system also displayed good anti-interference behavior with regar...
Source: Analytical and Bioanalytical Chemistry - Category: Chemistry Authors: Hong G, Zhang D, He Y, Yang Y, Chen P, Yang H, Zhou Z, Liu Y, Wang Y Tags: Anal Bioanal Chem Source Type: research