Vertically Aligned Multi-Walled Carbon Nanotubes based Flexible Immunosensor for extreme low level detection of Multidrug Resistant Leukemia Cells

We report an advanced flexible immunosensor for extreme low level detection of multidrug resistant myeloid leukemia cells. The detection mechanism is analyzed in terms of novel nanoscale interactions of target molecules with vertically aligned multi-walled carbon nanotubes (VA-MWNT). The immunosensor is fabricated by transferring VA-MWNTs on a polyethylene terephthalate substrate by hot press technique without losing CNTs’ pristine character. Sensing is performed using doxorubicin treated leukemia K562 cells in varying concentrations from 1.5 × 102 cells mL–1 to 1.5 × 107 cells mL–1 and sensor showed detection limit of 10 cells mL–1. The calibration plot of peak current versus logarithmic concentration of K562 cells exhibited good linearity with a regression coefficient of ∼0.98. Sensing mechanism is explained in terms of charge transfer induced Fermi level shift of sub µeV order, causing band bending at the interface of CNT-molecular species; and it is reflected in lowering detection limit of the fabricated sensor. Theoretical analysis is done to correlate Fermi energy shift with sensitivity of the device on cancer cell immobilization. Additionally, the developed immunosensor shows good stability, reproducibility and fast detection vis-à-vis the devices reported so far. The notable advantages of proposed flexible sensor are its durability, chemical and moisture resistance, making it a potentiall...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research

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Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
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Source: Journal of Pediatric Hematology Oncology - Category: Hematology Tags: Online Articles: Original Articles Source Type: research
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Source: Asian Pacific Journal of Cancer Prevention - Category: Cancer & Oncology Tags: Asian Pac J Cancer Prev Source Type: research
New Brunswick, N.J. (July 16, 2020) – Johnson &Johnson (NYSE: JNJ) today announced results for second-quarter 2020. “Our second quarter results reflect the impact of COVID-19 and the enduring strength of our Pharmaceutical business, where we saw continued growth even in this environment,” said Alex Gorsky, Chairman and Chief Executive Officer. “Thanks to the tireless work of our colleagues around the world and our broad range of capabilities, we continue to successfully navigate the external landscape, and we remain focused on advancing the development of a vaccine to help address this pandemic ...
Source: Johnson and Johnson - Category: Pharmaceuticals Source Type: news
a M. N. Abdelhafez A new series of methyl 2-(2-(4′-[2.2]paracyclophanyl)-hydrazinylidene)-3-substituted-4-oxothiazolidin-5-ylidene)acetates 3a–f were synthesized from the reaction of paracyclophanyl-acylthiosemicarbazides 2a–f with dimethyl acetylenedicarboxylate. Based upon nuclear magnetic resonance (NMR), infrared (IR), and mass spectra (HRMS), the structure of the obtained products was elucidated. X-ray structure analysis was also used as unambiguous tool to elucidate the structure of the products. The target compounds 3a–f were screened against 60 cancer cell lines. They dis...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
In this study, we investigated the modulatory potential of the CDK4/6 inhibitors abemaciclib, palbociclib, and ribociclib on AML resistance using peripheral blood mononuclear cells (PBMC) isolated from patients with de novo diagnosed AML. We first confirmed inhibitory effect of the tested drugs on ABCB1 and ABCG2 in ABC transporter-expressing resistant HL-60 cells while also showing the ability to sensitize the cells to cytotoxic drugs even as no effect on AML-relevant CRE isoforms was observed. All tested CDK4/6 inhibitors elevated mitoxantrone accumulations in CD34+ PBMC and enhanced accumulation of mitoxantrone was foun...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research
Abstract Conflicts with the notion that specific substrate interactions were required in the control of reaction path in active transport systems, P-glycoprotein showed extraordinarily low specificity. Therefore, overexpression P-glycoprotein excluded a large number of anticancer agents from cancer cells, and multidrug resistance happened. Several kinds of bisbenzylisoqunoline alkaloids were reported to modulate P-glycoprotein function and reverse drug resistance. In order to provide more information for their structure activity relationship on P-glycoprotein function, the effects of tetrandrine, isotetrandrine, f...
Source: Bioorganic and Medicinal Chemistry - Category: Chemistry Authors: Tags: Bioorg Med Chem Source Type: research
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Source: Journal of Biological Chemistry - Category: Chemistry Authors: Tags: Glycobiology and Extracellular Matrices Source Type: research
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Source: AAPS PharmSciTech - Category: Drugs & Pharmacology Authors: Tags: AAPS PharmSciTech Source Type: research
Abstract Multidrug resistance (MDR) constitutes the major cause of the failure in anticancer therapy. One of the most important mechanisms leading to the occurrence of MDR is related to the modulation of cellular death pathways. The aim of this study was to determine the effect of quercetin (Q) on triggering the programed death of human promyelocytic leukemia sensitive cells HL60 as well as multidrug resistant HL60/VINC cells overexpressing P-glycoprotein and HL60/MX2 cells characterized by the presence of mutated α isoform of topoisomerase II and the absence of β isoform of this enzyme. Q exerted compa...
Source: Nutrition and Cancer - Category: Cancer & Oncology Authors: Tags: Nutr Cancer Source Type: research
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