Analysis of Prenylflavonoids from Aerial Parts of Epimedium grandiflorum and Dietary Supplements using HPTLC, UHPLC-PDA and UHPLC-QToF along with Chemometric Tools to Differentiate Epimedium Species

Publication date: Available online 28 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ji-Yeong Bae, Bharathi Avula, Jianping Zhao, Vijayasankar Raman, Yan-Hong Wang, Mei Wang, Fazila Zulfiqar, Wei Feng, Jong Hee Park, Naohito Abe, Zulfiqar Ali, Ikhlas A. KhanABSTRACTAn UHPLC method was developed for the determination of 15 prenylflavonoids from aerial parts of Epimedium grandiflorum and related species (Berberidaceae). The separation was achieved using a reverse phased column and water/acetonitrile gradient as a mobile phase, and a temperature of 40°C. The developed analytical method was validated for linearity, limits of detection (LOD) and limits of quantification (LOQ), stability and repeatability. The LOD and LOQ were found to be in the range from 0.1-0.5 µg/mL and 0.3-1 µg/mL, respectively. The wavelength used for quantification with the photodiode array detector was 269 nm. The total content of 15 prenylflavonoids was 9.1-20.6 mg/g for E. grandiflorum (except for sample #2899 and #20862), 5.6-35.4 mg/g for E. brevicornu and 10.8-30.5 mg/g for E. sagittatum. Twenty dietary supplements contained compounds in the range from 0.1-81.7 mg/day. The developed method is simple, rapid and especially suitable for quality assessment of E. grandiflorum and dietary supplements containing E. grandiflorum. Liquid chromatography quadrupole time-of-flight-mass spectrometry (LC-QToF) is described for the identification and confirmation of co...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research