Crystal structure of TchmY from Actinoplanes teichomyceticus

Moenomycin-type antibiotics are phosphoglycolipids that are notable for their unique modes of action and have proven to be useful in animal nutrition. The gene clusters tchm from Actinoplanes teichomyceticus and moe from Streptomyces are among a limited number of known moenomycin-biosynthetic pathways. Most genes in tchm have counterparts in the moe cluster, except for tchmy and tchmz, the functions of which remain unknown. Sequence analysis indicates that TchmY belongs to the isoprenoid enzyme C2-like superfamily and may serve as a prenylcyclase. The enzyme was proposed to be involved in terminal cyclization of the moenocinyl chain in teichomycin, leading to the diumycinol chain of moenomycin isomers. Here, recombinant TchmY protein was expressed in Escherichia coli and its crystal structure was solved by SIRAS. Structural analysis and comparison with other prenylcyclases were performed. The overall fold of TchmY consists of an ( α / α )6-barrel, and a potential substrate-binding pocket is found in the central chamber. These results should provide important information regarding the biosynthetic basis of moenomycin antibiotics.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: crystal structure prenylcyclases Actinoplanes teichomyceticus research communications Source Type: research

Related Links:

Publication date: Available online 17 February 2020Source: Journal of Hazardous MaterialsAuthor(s): Baihui Xia, Fang Deng, Shuqu Zhang, Li Hua, Xubiao Luo, Meiying AoAbstractPetal-like ZnS-SnS2 heterojunctions with Z-scheme band alignment were prepared by one-pot solvothermal strategy. The optimal (1:1) ZnS-SnS2 can degrade 93.46% of tetracycline and remove 73.9% COD of pharmaceutical wastewater under visible-light irradiation due to the efficient production of •H, •O2-, h+ and •OH. The toxicity evaluation by ECOSAR prediction and the growth of E. coli indicates efficient toxicity reduction of tetracycline b...
Source: Journal of Hazardous Materials - Category: Environmental Health Source Type: research
Publication date: Available online 17 February 2020Source: International Journal of PharmaceuticsAuthor(s): Amin Afkhami-Poostchi, Mansour Mashreghi, Mehrdad Iranshahi, Maryam M. MatinAbstractBacteria-directed enzyme prodrug therapy (BDEPT), is an emerging alternative directed and tumor-specific approach. The basis of this method is the conversion of a non-toxic prodrug by a bacterial enzyme to a toxic drug within the tumor-microenvironment (TME). In the present study, the therapeutic efficacy of BDEPT was investigated based on the ability of Escherichia coli DH5α-lux/βG in activation of glycyrrhizic acid (GL), ...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research
Publication date: Available online 17 February 2020Source: International Journal of PharmaceuticsAuthor(s): Yadira Pastor, Isaiah Ting, Ana Luisa Martínez, Juan Manuel Irache, Carlos GamazoAbstractThermosensitive hydrogels have been studied as feasible needle-avoidance alternative to vaccine delivery. In this work, we report the development of a new thermal-sensitive hydrogel for intranasal vaccine delivery. This delivery system was formulated with a combination of the polymer Gantrez® AN119 and the surfactant Pluronic® F127 (PF127), with a high biocompatibility, biodegradability and immunoadjuvant propertie...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research
Publication date: May 2020Source: Materials Science and Engineering: C, Volume 110Author(s): Haixun Ji, Shengnan Zhou, Yuqin Fu, Yan Wang, Jiaoyang Mi, Tiancheng Lu, Xiuran Wang, Changli LüAbstractThe emergence of bacterial resistance has become one of the top global concern, and silver nanoparticles (AgNPs) provide alternative strategies for the development of new antimicrobial agent. Herein, three small sizes (1.5–4.0 nm) of well-dispersed AgNPs were successfully synthesized using a thermo-sensitive P(NIPAM-co-MQ) copolymer with coordination ability as a stabilizer. The copolymer stabilized silver nanopar...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
LangmuirDOI: 10.1021/acs.langmuir.9b03606
Source: Langmuir - Category: Chemistry Authors: Source Type: research
Source: Infection and Drug Resistance - Category: Infectious Diseases Tags: Infection and Drug Resistance Source Type: research
In conclusion, the results showed that foods of animal origin must be regarded as a reservoir of ESBL-producing bacteria of clinical relevance, which might spread through the food chain.
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
Analyst, 2020, Accepted Manuscript DOI: 10.1039/D0AN00224K, PaperYanan Zhao, Dexin Zeng, Chao Yan, Wei Chen, Jianluan Ren, Yuan Jiang, Luyan Jiang, Feng Xue, Dejun Ji, Fang Tang, Mingqiang Zou, Jianjun Dai Escherichia coli O157:H7 is a severe foodborne pathogen that causes lots of life-threatening diseases. To search for a rapid, sensitive, portable and low-cost method detecting this pathogen, we developed a... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Analyst latest articles - Category: Chemistry Authors: Source Type: research
Shine-Dalgarno (SD) motifs are thought to play an important role in translational initiation in bacteria. Paradoxically, ribosome profiling studies inE. coli show no correlation between the strength of an mRNA's SD motif and how efficiently it is translated. Performing profiling on ribosomes with altered anti-Shine-Dalgarno sequences, we reveal a genome-wide correlation between SD strength and ribosome occupancy that was previously masked by other contributing factors. Using the antibiotic retapamulin to trap initiation complexes at start codons, we find that the mutant ribosomes select start sites correctly, arguing that ...
Source: eLife - Category: Biomedical Science Tags: Chromosomes and Gene Expression Source Type: research
Source: Infection and Drug Resistance - Category: Infectious Diseases Tags: Infection and Drug Resistance Source Type: research
More News: Biochemistry | Gastroenteritis | Genetics | Nutrition