Chemosensitizing effect of cernumidine extracted from Solanum cernuum Vell. on bladder cancer cells in vitro.

Chemosensitizing effect of cernumidine extracted from Solanum cernuum Vell. on bladder cancer cells in vitro. Chem Biodivers. 2019 Aug 25;: Authors: Bastos J, Miranda MA, Mondal A, Sachdeva M, Cabral H, Neto YAAH, Khan I, Groppo M, McChesney JD Abstract Cernumidine (CER) is a guanidinic alkaloid isolated from Solanum cernuum's leaves. In this work, we investigated the cytotoxicity, chemosensitizing effect of cernumidine to cisplatin (cDDP) and the possible mechanism of action of the combination on bladder cancer cells. Cernumidine showed cytotoxicity and could sensitize bladder cancer cells to cisplatin. The combination of CER+cDDP inhibited cell migration on T24 cells. CER+cDDP down-regulated MMP-2/9 and p-ERK1/2, while it increased EGFR activity corroborating the observed cell migration inhibition. Down-regulation of Bcl-2 and up-regulation pro-apoptotic Bax and further depletion of the mitochondrial membrane potential (ΔΨm) indicates that mitochondria play a central role in the combination treatment inducing the mitochondrial signaling pathway of apoptosis in T24 cells. Our data showed that the alkaloid cernumidine is worthy of further studies as a chemosensitizing agent to be used in complementary chemotherapy. PMID: 31448497 [PubMed - as supplied by publisher]
Source: Chemistry and Biodiversity - Category: Biochemistry Authors: Tags: Chem Biodivers Source Type: research

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Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
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Source: Urologic Oncology - Category: Urology & Nephrology Authors: Tags: Urol Oncol Source Type: research
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Source: Urologic Oncology - Category: Urology & Nephrology Authors: Tags: Urol Oncol Source Type: research
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Source: Urology - Category: Urology & Nephrology Authors: Source Type: research
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