1023 - regeneration of t lymphopoiesis from pluripotent stem cells by defined factors

Regarding the unlimited source and gene-editing advantages of pluripotent stem cells (PSC), generating functional T lymphocytes from PSC provides an ideal approach for broadening T cell-based translational applications. In the presence of Notch ligands, PSC can conventionally generate induced T cell progenitors, which reportedly lack thymus-homing capacity in vivo. Using an inducible tandem-knock-in strategy and a functional screening by transplantation setting, we screened the combinatory effects of the essential transcription factors, which are abundantly expressed in developmental pre-HSC but absent in induced hemogenic endothelial cells (CD45 −CD41+CD31+CD201high, HEC), in generating functional T lymphopoiesis in vivo.

Source: Experimental Hematology - July 31, 2019 Category: Hematology Authors: Jinyong Wang, Rongqun Guo, Fangxiao Hu, Qitong Weng, Cui Lv, Hongling Wu, Lijuan Liu, Bing Liu Source Type: research