Paradoxical reactions in Buruli ulcer after initiation of antibiotic therapy: Relationship to bacterial load

by Michael Frimpong, Bernadette Agbavor, Mabel Sarpong Duah, Aloysius Loglo, Francisca N. Sarpong, Justice Boakye-Appiah, Kabiru M. Abass, Mathias Dongyele, George Amofa, Wilson Tuah, Margaret Frempong, Yaw A. Amoako, Mark Wansbrough-Jones, Richard O. Phillips BackgroundWe investigated the relationship between bacterial load in Buruli ulcer (BU) lesions and the development of paradoxical reaction following initiation of antibiotic treatment. MethodsThis was a longitudinal study involving BU patients from June 2013 to June 2017. Fine needle aspirates (FNA) and swab samples were obtained to establish the diagnosis of BU by PCR. Additional samples were obtained at baseline, during and after treatment (if the lesion had not healed) for microscopy, culture and combined 16S rRNA reverse transcriptase/ IS2404 qPCR assay. Patients were followed up at regular intervals until complete healing. ResultsForty-seven of 354 patients (13%) with PCR confirmed BU had a PR, occurring between 2 and 42 (median 6) weeks after treatment initiation. The bacterial load, the proportion of patients with positiveM.ulcerans culture (15/34 (44%) vs 29/119 (24%), p = 0.025) and the proportion with positive microscopy results (19/31 (61%) vs 28/90 (31%), p = 0.003) before initiation of treatment were significantly higher in the PR compared to the no PR group. Plaques (OR 5.12; 95% CI 2.26 –11.61;p
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research