Molecules, Vol. 24, Pages 3088: Evaluation of Tryptic Podocin Peptide in Urine Sediment Using LC-MS-MRM Method as a Potential Biomarker of Glomerular Injury in Dogs with Clinical Signs of Renal and Cardiac Disorders
Molecules, Vol. 24, Pages 3088: Evaluation of Tryptic Podocin Peptide in Urine Sediment Using LC-MS-MRM Method as a Potential Biomarker of Glomerular Injury in Dogs with Clinical Signs of Renal and Cardiac Disorders
Molecules doi: 10.3390/molecules24173088
Authors:
Barbara Szczepankiewicz
Remigiusz Bąchor
Robert Pasławski
Natalia Siwińska
Urszula Pasławska
Andrzej Konieczny
Zbigniew Szewczuk
The early asymptomatic stage of glomerular injury is a diagnostic challenge in the course of renal and extra-renal disease, e.g., heart insufficiency. It was found that podocin, a podocyte-specific protein present in the urine, may serve as a biomarker in the diagnosis of glomerular disease in humans and animals including glomerulonephritis, glomerulosclerosis, amyloidosis, or nephropathy. Therefore, there is a need of development of the sensitive and straightforward method of urinary podocin identification. In this work, we report our extended research under the glomerular injury investigation in dogs by application of clinical examination and LC-MS-MRM method in the identification of canine podocin in urine samples. The LC-MS-MRM method is based on the identification of podocin tryptic peptide with the 218H-AAEILAATPAAVQLR-OH232 sequence. The model peptide was characterized by the highest ionization efficiency of all the proposed model podocin tryptic peptides in a canine urine sediment according to the LC-MS/MS analysis. The obtained results revealed the presenc...
Source: Molecules - Category: Chemistry Authors: Barbara Szczepankiewicz Remigiusz B ąchor Robert Pas ławski Natalia Siwi ńska Urszula Pas ławska Andrzej Konieczny Zbigniew Szewczuk Tags: Article Source Type: research
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