Chloroplast-based inducible expression of ESAT-6 antigen for development of a plant-based vaccine against tuberculosis

Publication date: Available online 24 August 2019Source: Journal of BiotechnologyAuthor(s): Kiran Saba, Johanna Gottschamel, Iqra Younus, Tahira Syed, Kehkshan Gull, Andreas Günter Lössl, Bushra Mirza, Mohammad Tahir WaheedAbstractMycobacterium tuberculosis causes tuberculosis in humans. The major disease burden of tuberculosis lies in developing countries. Lack of an effective vaccine for adults is one of the major hurdles for controlling this deadly disease. In the present study, 6 kDa early secretory antigenic target (ESAT-6) of M. tuberculosis was inducibly expressed in chloroplasts of Nicotiana tabacum. The expression of ESAT-6 in chloroplasts was controlled by T7 promoter that was activated by nuclear-generated signal peptide. Tobacco plants, containing nuclear component, were transformed via biolistic bombardment with pEXP-T7-ESAT-6 obtained by Gateway® cloning. Transformation and homoplasmic status of transplastomic plants was confirmed by polymerase chain reaction and Southern blotting. Plants were induced for protein expression by spraying with 5% ethanol for 1 day, 3 days, 7 days and 10 days. ESAT-6 protein was detected by immunoblot analysis and maximum protein was obtained for 10 days induced plants that was estimated to accumulate up to 1.2% of total soluble fraction of protein. Transplastomic plants showed completely normal morphology. Transplastomic and untransformed plants became slightly chlorotic upon prolonged exposure to ethanol until...
Source: Journal of Biotechnology - Category: Biotechnology Source Type: research

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The 20th International Leprosy Congress (ILC) is being held Sept. 10 to 13 in Manila, Philippines. The conference is hosted every three years and was last held in China in 2016. Credit: Stella Paul/IPS By Stella PaulMANILA, Sep 12 2019 (IPS) Rachna Kumari of Munger in Eastern India’s Bihar state is not yet 30. But she’s already been married at 18, abandoned by her husband after she was diagnosed with leprosy and become an award-winning advocate of the disease. She has traversed a long road. And this week she undertook another step in her journey to fly to Manila, Philippines, as a delegate at the 20th Internati...
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Melioidosis is a tropical disease that claims an estimated 90,000 lives worldwide each year. There is no vaccine, and current treatments are hampered by the ability of the bacterium that causes the disease to resist even the strongest antibiotics.Hardy and lethal, that bacterium, Burkholderia pseudomallei, is classified by the Centers for Disease Control and Prevention as a potential bioweapon.UCLA-led research has identified two compounds that, based on tests on human cells and on mice, show potential for treating melioidosis. One is a widely used drug already approved by the U.S. Food and Drug Administration as an antifu...
Source: UCLA Newsroom: Health Sciences - Category: Universities & Medical Training Source Type: news
Glycolipids of the cell wall of Mycobacterium tuberculosis (Mtb) are important immunomodulators in tuberculosis. In particular, lipoarabinomannan (LAM) has a profound effect on the innate immune response. LAM and its structural variants can be recognized by and activate human CD1b-restricted T cells, and emerging evidence indicates that B cells and antibodies against LAM can modulate the immune response to Mtb. Anti-LAM antibodies are induced during Mtb infection and after bacille Calmette–Guerin (BCG) vaccination, and monoclonal antibodies against LAM have been shown to confer protection by passive administration in...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Discussion With continued exposure to respiratory pathogens and the emergence of novel respiratory pathogens, personal protective equipment (PPE) and procedures are important for decreasing occupational exposure to respiratory pathogens. PPE and procedures are particularly important when anti-infective treatments or vaccination are unavailable or have limited effectiveness. Medical masks are “[a]lso known as a surgical or procedure mask. As personal protective equipment, a facial mask is intended to protect caregivers and health-care workers against droplet-transmitted pathogens, or to serve as part of facial protect...
Source: PediatricEducation.org - Category: Pediatrics Authors: Tags: Uncategorized Source Type: news
Vaccine development against tuberculosis (TB) is based on the induction of adaptive immune responses endowed with long-term memory against mycobacterial antigens. Memory B and T cells initiate a rapid and robust immune response upon encounter with Mycobacterium tuberculosis, thus achieving long-lasting protection against infection. Recent studies have shown, however, that innate immune cell populations such as myeloid cells and NK cells also undergo functional adaptation after infection or vaccination, a de facto innate immune memory that is also termed trained immunity. Experimental and epidemiological data have shown tha...
Source: Journal of Clinical Investigation - Category: Biomedical Science Authors: Source Type: research
Conclusion: The M72/AS01E vaccine against TB is safe and effective. Although the vaccine is associated with a mild adverse reaction, it is promising for the prevention of TB in healthy adults. Keywords: vaccine, tuberculosis, M72/AS01E, immunogenicity, safety
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
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Source: bizjournals.com Health Care:Biotechnology headlines - Category: Biotechnology Authors: Source Type: news
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Source: PharmaManufacturing.com - Category: Pharmaceuticals Source Type: news
Conditions:   Tuberculosis;   BCG Vaccination Interventions:   Device: BCG vaccination with MicronJet600;   Device: BCG vaccination with conventional needle Sponsor:   Yonsei University Enrolling by invitation
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
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