Flavonoids from Rhynchosia minima root exerts anti-inflammatory activity in lipopolysaccharide-stimulated RAW 264.7 cells via MAPK/NF- κB signaling pathway

In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) fromR. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism. The compound composition of EEF6 was determined by high-performance liquid chromatography-mass spectrometry. The result showed that five flavonoids compounds, 2 ′,4′,5,7-tetrahydroxyisoflavone, genistein-8-C-glucopyranoside, tricin, genistein, and daidzein, were identified in EEF6. In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF- κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38. These results indicated that EEF6 co uld be a promising ingredient for inflammation management.
Source: Inflammopharmacology - Category: Drugs & Pharmacology Source Type: research

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We report on a family of three in a rabbit farm, especially children with Majocchi granuloma as the main clinical feature of cross-infection of Trichophyton mentagrophytes, provides experience in reducing the rate of clinical misdiagnosis. Based on the pathological features of Majocchi's granuloma, taking into account that severe inflammatory reactions are also responsible for the damage of the body. In Majocchi's granuloma treatment strategy, we recommend the use of anti-inflammatory Chinese medicines that have a hormonal effect and have no side effects.
Source: Medical Mycology Case Reports - Category: Biology Source Type: research
In this study, we used the Megalobrama amblycephala to investigate the effects of G. elegans alkaloids on fish intestinal health after diet supplementation with 0, 5, 10, 20 and 40 mg/kg G. elegans alkaloids for 12 weeks. We found that dietary G. elegans alkaloids at 40 mg/kg improved intestinal morphology by increasing villus length, muscle thickness and villus number in the foregut and midgut and muscle thickness in the hindgut (P 
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
In conclusion, the results provided further insight into the immune roles of galectin-1 in teleost.
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
Publication date: Available online 21 September 2019Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Ping Wai Man, Annemieke C. Heijboer, Irene M. van der Meer, Wenzhi Lin, Mattijs E. Numans, Paul Lips, Barend J.C. Middelkoop, Daan J. TouwAbstractBlood for determining 25-hydroxyvitamin D3 [25(OH)D3] is usually obtained through venipuncture although, as an alternative for serum, dried blood spot (DBS) can be considered. The aim of this proof-of-concept study was to investigate levels of agreement between measurements of 25(OH)D3 obtained with DBS compared with serum. 301 Chinese participants were ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - Category: Biochemistry Source Type: research
Publication date: Available online 21 September 2019Source: Plant Physiology and BiochemistryAuthor(s): Afef Ladhari, Joe ChappellAbstractCleome arabica is a medicinal plant contains diverse bioactive compounds and terpenoids are the major components. However, the isolation and purification of the active triterpenes from this plant involve long and complicated procedures. The present work investigates the triterpenes profiles of different tissues, besides that, describes the isolation, heterologous expression and functional characterization of C. arabica gene coding for triterpenes synthases. The phytochemical investigatio...
Source: Plant Physiology and Biochemistry - Category: Biochemistry Source Type: research
Publication date: December 2019Source: Phytochemistry, Volume 168Author(s): Zengyuan Wang, Mengjun Jiang, Afsar Khan, Shengbao Cai, Xiaonian Li, Junqiu Liu, Guoyin Kai, Tianrui Zhao, Guiguang Cheng, Jianxin CaoAbstractFive undescribed C21 pregnane glycosides, epigycosides D−H, together with four known analogues, two lignans, and a flavonoid have been isolated from the stems of Epigynum cochinchinensis. The structures of pregnane glycosides were elucidated using spectroscopic techniques and acid hydrolysis. The in vitro immunological activities were assessed against Con A-stimulated proliferation of mice splenocytes. ...
Source: Phytochemistry - Category: Chemistry Source Type: research
Publication date: Available online 21 September 2019Source: Chinese Chemical LettersAuthor(s): Rui Guo, Tianming Lv, Fengying Han, Zilin Hou, Guodong Yao, Bin Lin, Xiaobo Wang, Xiaoxiao Huang, Shaojiang SongABSTRACT(±)-Crataegusnorin A (1a/1b) and B (2a/2b), two pairs of rare 8,9′-epoxy-type norlignan enantiomers featuring a γ-butyrolactone ring, were isolated from the fruit of Crataegus pinnatifida. Their structures were determined via extensive spectroscopic analyses. Gauge-independent atomic orbital (GIAO) NMR chemical shift calculations, combined with the advanced statistical method DP4+ were employe...
Source: Chinese Chemical Letters - Category: Chemistry Source Type: research
Conclusion The elucidation of the function of circRNAs is an emerging field of science with a tremendous potential after previously being dismissed as RNA artifacts. They are ubiquitously expressed and thousands of members have already been identified. This fact only expands their potential to possibly enhance our knowledge to understand the difference between health and disease. Owing to their structure stability and their presence in exosomes circRNAs may also exert their function in an autocrine, paracrine and possible endocrine fashion. In addition, the fact that circRNAs are widely distributed in the cellular compart...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
Breast implant–associated lymphoma has recently gained wide recognition. Anaplastic large cell lymphoma (ALCL) is the most frequently diagnosed subtype in this setting but the spectrum is broadening. A 66-year-old woman developed swelling and itch around her saline implant 6 years after its insertion. Imaging revealed a fluid collection surrounding the implant with an adjacent mass. Microscopy showed sclerotic tissue punctuated by discrete cellular nodules comprising small lymphocytes, eosinophils and interspersed large atypical Hodgkin Reed-Sternberg (HRS)-like cells. The HRS-like cells stained positively for CD30 a...
Source: International Journal of Surgical Pathology - Category: Pathology Authors: Tags: Case Reports Source Type: research
Pefani et al. identify that the tumor suppressor RASSF1A is an integral component in TGF-β signaling. TGF-β stimulates RASSF1A recruitment to TGF-βRI at the cell membrane, where it is targeted for degradation by the E3 ligase ITCH. Degradation of RASSF1A allows YAP/SMAD interaction, nuclear localization, and TGF-β−mediated transcription.
Source: Molecular Cell - Category: Cytology Authors: Tags: Short Article Source Type: research
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