Evolution of multi-functional capillary electrophoresis for high-efficiency selection of aptamers.

Evolution of multi-functional capillary electrophoresis for high-efficiency selection of aptamers. Biotechnol Adv. 2019 Aug 19;:107432 Authors: Zhu C, Yang G, Ghulam M, Li L, Qu F Abstract Aptamers have drawn considerable attention as newly emerging molecular recognition elements in clinical diagnostics, drug delivery, therapeutics, environmental monitoring, and food safety analyses. As the in vitro screening antibody analogs, aptamers are enabled to recognize various types of targets with high affinity and specificity like or even superior to antibodies. However, the restrictions and inefficiency of selection have been hampering their wider application. Among various modified systematic evolution of ligands by exponential enrichment (SELEX) methods, capillary electrophoresis (CE)-SELEX holds multiple functions and advantages with the powerful qualitative and quantitative analysis capabilities, less consumption of sample and analytical reagent, natural binding environment, higher screening efficiency, and availability in multiple modes. This review summarizes the key developments in the area of CE-SELEX by leading research groups, including our teams' ten years of research and experience to help researchers fully understand and utilize CE-SELEX. Aptamers' history, applications, as well as the SELEX developments, have been briefly described; the advantages of CE-SELEX are highlighted compared with the conventional SELEX methods. Furth...
Source: Biotechnology Advances - Category: Biotechnology Authors: Tags: Biotechnol Adv Source Type: research