Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus

Publication date: 1 December 2019Source: Carbohydrate Polymers, Volume 225Author(s): Ruberney S. Oliveira, Stellee M.P. Biscaia, Daniel L. Bellan, Sthefany R.F. Viana, Maria Carolina Di-Medeiros Leal, Ana Flora D. Vasconcelos, Luciano M. Lião, Edvaldo S. Trindade, Elaine R. CarboneroAbstractA fucomannogalactan (FMG-Hm), with a molecular weight of 17.1 kDa, obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects. FMG-Hm was not cytotoxic, nor did it alter the cell morphology and proliferation, but was able to inhibit colony-forming ability and cell migration in B16-F10 murine melanoma cells. An analysis of the monosaccharide composition indicated that FMG-Hm was composed of fucose, mannose, and galactose in a ratio of 1.00:1.08:3.17. The FMG-Hm was structurally characterized based on methylation analysis, partial acid hydrolysis, and NMR experiments. The results indicated that FMG-Hm contained a α-(1→6)-linked galactopyranosyl main chain, partially substituted at O-2 by non-reducing ends of α-L-fucopyranose and β-D-mannopyranose. The predicted structure of the heteropolysaccharide was established as:
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research