Sex variation in hematological and serum biochemical parameters of cultured Chinese silver carp, Hypophthalmichthys molitrix

AbstractHematological parameters are essential for the evaluation of both physiological and pathological changes developed in fishes due to the involvement of endogenous and exogenous factors. Among the endogenous factors, sex plays a significant role in determining the hematological and serum biochemical parameters of fish. The aim of the present study was to obtain a basic knowledge about the variation in hematological and serum biochemical parameters of cultured silver carp,Hypophthalmichthys molitrix with respect to sex. In order to establish the information, a blood sample was collected from the fishes from March to June 2018 and were analyzed by using standard techniques. Hematological parameters such as hemoglobin concentration (Hb), total red blood (RBC), total white blood cell (WBC) counts, hematocrit value (Hct), erythrocyte sedimentation rate (ESR), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and mean corpuscular volume (MCV) were estimated. Hb and Hct were analyzed by using cyanmethemoglobin and microhaematocrit methods, while total RBC and WBC counts were obtained by using the Neubauer hemocytometer. The biochemical parameters total protein, albumin, globulin, glucose, cholesterol and aspartate aminotransferase (AST) were analyzed by means of commercial kits. Data were subjected to statistical analysis which revealed that significant (P <  0.05) differences were observed in Hb, RBC, WBC and Hct values between the sexe...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research