Proresolving mediators LXB4 and RvE1 regulate inflammation in stromal cells from patients with shoulder tendon tears.

Proresolving mediators LXB4 and RvE1 regulate inflammation in stromal cells from patients with shoulder tendon tears.
Am J Pathol. 2019 Aug 19;:
Authors: Dakin SG, Colas RA, Wheway K, Watkins B, Appleton L, Rees J, Gwilym S, Little C, Dalli J, Carr AJ
Abstract
Tendon stromal cells isolated from patients with chronic shoulder rotator-cuff tendon tears show dysregulated resolution responses. Current therapies do not address the biological processes concerned with persistent tendon inflammation; therefore, new therapeutic approaches targeting tendon stromal cells are required. We determined if two specialized pro-resolving mediators (SPM) LXB4 and RvE1, modulate the bioactive lipid mediator profiles of interleukin (IL)-1β-stimulated tendon cells derived from patients with shoulder tendon tears and healthy volunteers. We also determined if LXB4 or RvE1 treatments moderated the pro-inflammatory phenotype of tendon tear stromal cells. Incubation of IL-1β-treated patient-derived tendon cells in LXB4 or RvE1 up-regulated concentrations of SPM. RvE1 treatment of diseased tendon stromal cells increased 15-epi-LXB4 and regulated PGF2α. LXB4 or RvE1 also induced expression of the SPM biosynthetic enzymes 12-liopxygeanse (ALOX12), and ALOX15. RvE1 treatment up-regulated proresolving receptor ERV1 compared to vehicle-treated cells. Incubation in LXB4 or RvE1 moderated the proinflammatory phenotype of patient-derived tendon tear cells, regulatin...

Source: The American Journal of Pathology - August 18, 2019 Category: Pathology Authors: Dakin SG, Colas RA, Wheway K, Watkins B, Appleton L, Rees J, Gwilym S, Little C, Dalli J, Carr AJ Tags: Am J Pathol Source Type: research