Anti-diabetic effects of a phenolic-rich extract from Hypericum attenuatum Choisy in KK-Ay mice mediated through AMPK /PI3K/Akt/GSK3β signaling and GLUT4, PPARγ, and PPARα expression

In this study, we investigated anti-diabetic activities of a phenolic-rich extract from Hypericum attenuatum Choisy (HA-PRE). In vivo, 4-week treatment of HA-PRE improved hyperglycemia, obesity, glucose tolerance, and hyperinsulinism. Western blot analysis revealed that HA-PRE increased AMPKα and Akt phosphorylation in skeletal muscle, liver and white adipose tissue (WAT); increased GSK3β phosphorylation in skeletal muscle and liver; and enhanced GLUT4 expression in WAT and skeletal muscle. HA-PRE reduced PPARγ expression, increased PPARα expression, and enhanced ACC phosphorylation in liver and WAT. In vitro, HA-PRE enhanced glucose uptake. Mechanisms analysis revealed that HA-PRE increased GLUT4, p-AMPKα, p-Akt, and p-GSK3β expression in L6 myotubes. Moreover, Compound C, an AMPK inhibitor, partly inhibited these effects. Wortmannin, a PI3K inhibitor, inhibited HA-PRE-stimulated Akt phosphorylation and GSK3β phosphorylation, but had no effects on AMPKα phosphorylation. Taken together, these findings indicate that HA-PRE alleviate diabetes through AMPK/PI3K/Akt/GSK3β and regulating GLUT4, PPARα, and PPARγ expression.Graphical abstract
Source: Journal of Functional Foods - Category: Nutrition Source Type: research

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Conclusion Our study provides novel evidence that EXE-induced antisenescence against metabolic distress in the hippocampus may be a key neuroprotective mechanism, preventing neuroinflammation and oxidative stress.
Source: Medicine and Science in Sports and Exercise - Category: Sports Medicine Tags: BASIC SCIENCES Source Type: research
Purpose Low-attenuation muscle (LAM) area at mid-thigh, a computed tomography (CT)–derived index of intramuscular lipids, is associated with insulin resistance, obesity, and type 2 diabetes. The present study aimed at testing the hypothesis that changes in LAM area in trunk muscles from a single abdominal scan could provide relevant information to evaluate the effects of a lifestyle intervention without the use of a mid-thigh CT scan. Methods Cardiometabolic risk variables, including waist circumference, lipoprotein–lipid profile, glucose tolerance, and cardiorespiratory fitness, were assessed in a sample ...
Source: Medicine and Science in Sports and Exercise - Category: Sports Medicine Tags: APPLIED SCIENCES Source Type: research
i-FectTM Delivers Again!Research shows that rats and humans on a high-fat diet (HFD) are less sensitive to satiety signals known to act via vagal afferent pathways. Impaired vagal afferent responsiveness to both gastric satiety hormones (CCK and leptin) and mechanical stimulation raises the possibility that changes in electrophysiological properties may be the underlying mechanism responsible for impaired vagal responsiveness to a wide variety of satiety signals.Potassium channels play a central role. To demonstrate this researchers used ouri-Fect siRNA Transfection Kit to silence TRESK and TASK1 to understand there impact...
Source: Neuromics - Category: Neuroscience Tags: diabetes iFect obesity Obesity Research RNAi siRNA siRNA delivery in-vivo Source Type: news
"These latest data shout that our national obesity crisis is getting worse," says the CEO of a nonprofit organization that publishes an annual report on the state of obesity in America.Medscape Medical News
Source: Medscape Medical News Headlines - Category: Consumer Health News Tags: Diabetes & Endocrinology News Source Type: news
2019 NIDDK Administrative Speaker Series The series is an opportunity for NIDDK staff to learn about the important science NIDDK conducts and supports. It is also an excellent way to inspire our administrative staff, as it allows them a chance to hear and see how their roles contribute to and/or impact the work of the scientists.Air date: 10/9/2019 1:00:00 PM
Source: Videocast - All Events - Category: General Medicine Tags: Upcoming Events Source Type: video
Research presented at the Annual Meeting of the European Association for the Study of Diabetes in Barcelona has linked obesity to a nearly 6-fold increased risk of developing type 2 diabetes, and proves the most dominant risk factor over others such as genetics and lifestyle.Medical Xpress
Source: Society for Endocrinology - Category: Endocrinology Source Type: news
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Source: OnMedica Latest News - Category: UK Health Source Type: news
Publication date: November 2019Source: Journal of Functional Foods, Volume 62Author(s): Ruiyu Wu, Xin Tang, Xincong Kang, Yushuang Luo, Lei Wang, Jian Li, Xiyang Wu, Dongbo LiuAbstractNumerous microbiotas, which play important roles in health, inhabit the human gut. A traditional Chinese medicine dietary therapy (TCMDT) diet, consisted of whole grains and traditional Chinese medicines, is traditionally used to lessen hyperglycemia and reduce obesity. The Simulator of the human intestinal microbial ecosystem (SHIME) was used for studying the effect of the TCMDT diet on gut microbiota and short chain fatty acids. The TCMDT d...
Source: Journal of Functional Foods - Category: Nutrition Source Type: research
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Source: Primary Care Diabetes - Category: Primary Care Authors: Tags: Original research Source Type: research
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Source: Molecular Genetics & Genomic Medicine - Category: Genetics & Stem Cells Authors: Tags: ORIGINAL ARTICLE Source Type: research
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