Strontium ‑containing α‑calcium sulfate hemihydrate promotes bone repair via the TGF‑β/Smad signaling pathway.

Strontium‑containing α‑calcium sulfate hemihydrate promotes bone repair via the TGF‑β/Smad signaling pathway. Mol Med Rep. 2019 Aug 20;: Authors: Liu Z, Yu Z, Chang H, Wang Y, Xiang H, Zhang X, Yu B Abstract Calcium phosphate‑based bone substitutes have been widely used for bone repair, augmentation and reconstruction in bone implant surgery. While some of these substitutes have shown excellent biological efficacy, there remains a need to improve the performance of the current calcium phosphate‑based bone substitutes. Strontium ions (Sr) can promote new osteogenesis, inhibit osteoclast formation and increase osteoconductivity. However, the therapeutic effect and mechanism of strontium‑containing α‑calcium sulfate hemihydrate (Sr‑CaS) remains unclear. The present study created bone injuries in rats and treated the injuries with Sr‑CaS. Then Cell Counting Kit‑8, soft agar colony formation, flow cytometry, Transwell and Alizarin Red staining assays were performed to assess the bone cells for their proliferation, growth, apoptosis, invasion, and osteogenic differentiation abilities. The bone reconstructive states were measured by the microCT method, hematoxylin and eosin staining and Masson staining. Bone‑related factors were analyzed by the reverse transcription‑quantitative PCR assay; transforming growth factor (TGF)‑β, mothers against decapentaplegic homolog (Smad)2/3 and β‑catenin expression was measu...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research