Metal-organic frameworks for on-demand pH controlled delivery of vancomycin from chitosan scaffolds

Publication date: December 2019Source: Materials Science and Engineering: C, Volume 105Author(s): Ayşe Karakeçili, Berna Topuz, Serdar Korpayev, Mert ErdekAbstractA potential bone substitute and drug carrier system is prepared to be used in treatment of serious bone infections like osteomyelitis. Vancomycin (VAN), as an antibiotic was loaded into ZIF8 nanocrystals for a pH responsive controlled release (ZIF8/VAN). Chitosan scaffolds loaded with ZIF8/VAN were prepared by wet-spinning to obtain 3D biocompatible scaffolds. Characterization of scaffolds were performed to determine the morphology, swelling behavior and pH controlled VAN release. Antibacterial activity studies were done to investigate the effectiveness of the carrier system against Staphylococcus aureus. VAN molecule encapsulation efficiency for nanosized ZIF8 crystals was calculated as 99.3%. The results showed that the VAN loaded to ZIF8 nanocrystals was released in a pH controlled manner from the chitosan scaffolds. About 70% of the VAN was released during 8 h at pH 5.4, while this value was 55% at pH 7.4. VAN release was increased with higher dissolution of ZIF8 in acidic conditions and reached a plateau value of ~77% at the end of 48 h at pH 5.4 conditions. ZIF8 and ZIF8/VAN chitosan scaffolds showed a strong effect in the reduction of S. aureus activity in comparison to chitosan scaffolds alone. This effect was best pronounced under pH 5.4 conditions which can mimic the environment of an i...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research

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CONCLUSION: Our study highlighted that the differences exist between the OAI and NIRI, specifically in terms of clinical features, distribution of luk genes, treatment approach, and outcome. Risk factors for both types of infection remained the same. PMID: 32960738 [PubMed - as supplied by publisher]
Source: Pathogens and Global Health - Category: International Medicine & Public Health Tags: Pathog Glob Health Source Type: research
Abstract We applied combination antibiotic therapy to treat vertebral osteomyelitis and a psoas abscess caused by glycopeptide-intermediate (MIC, 2 μg/ml) and daptomycin-non-susceptible (>2 μg/ml) methicillin-resistant Staphylococcus aureus The Etest synergy test showed the largest synergistic effects for imipenem/cilastatin and fosfomycin. Whole-gene sequencing showed amino acid changes in SA0802, SA1193 (mprF), and SA1531 (ald). Four weeks of combination treatment using imipenem/cilastatin (1.5 g per day) and fosfomycin (4.0 g per day) resulted in clinical improvement. PMID: 32958715 [PubMed - as s...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
Conclusion: These negative results are, however, relevant to report as they may save other research groups from conducting the same animal experiments and provide a platform for developing and evaluating other new potential tracers or protocol instead.
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Abstract Osteomyelitis is a bone marrow infection which generally involves cortical plates and which may occur after bone trauma, orthopedic/maxillofacial surgery or after vascular insufficiency episodes. It mostly affects people from the Third World Countries, elderly and patients affected by systemic diseases e.g. autoimmune disorders, AIDS, osteoporosis and microvascular disease. The highest percentage of osteomyelitis cases (almost 75%) is caused by Staphylococcus spp., and in particular by Staphylococcus aureus (more than 50%). The ideal classification and the diagnosis of osteomyelitis are two important tool...
Source: Current Drug Delivery - Category: Drugs & Pharmacology Authors: Tags: Curr Drug Deliv Source Type: research
CONCLUSION: IP vancomycin was effective for treatment of invasive S. aureus infection in this case. This approach should be considered in patients undergoing PD for whom peripheral i.v. access options are limited and/or not preferred. PMID: 32914168 [PubMed - as supplied by publisher]
Source: American Journal of Health-System Pharmacy : AJHP - Category: Drugs & Pharmacology Authors: Tags: Am J Health Syst Pharm Source Type: research
We report on a case of osteomyelitis involving the hip joint with thromblophlebitis complicated by necrotizing pneumonia and life-threatening septic shock. The child required advance respiratory support for 14 days with circulatory support for 7 days in ICU (intensive care unit), surgical draninage via arthrotomy of hip joint and second-line antibiotic treatment for 1 month. Among a wide literature, in Europe over half of Panton-Valentine St. Aureus (PVL-SA) is MSSA. Investigations for PVL are not always available determining an under-recognition of the episodes. Data on prevalence of PVL-SA in Italy are scarce. With this ...
Source: Journal of Biological Regulators and Homeostatic Agents - Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research
The objective of this retrospective study was to determine risk factors for and compare outcome differences between de novo methicillin-resistantStaphylococcus aureus (MRSA) VO and methicillin-sensitiveStaphylococcus aureus (MSSA) VO. A retrospective cohort study was performed by review of the electronic medical records of 4541 consecutive spine surgery patients. Among these 37 underwent surgical treatment of de novo MRSA and MSSA spinal infections. Patient demographics, pre- and postoperative neurological status (ASIA impairment score), surgical treatment, inflammatory laboratory values, nutritional status, comorbidities,...
Source: Neurosurgical Review - Category: Neurosurgery Source Type: research
Staphylococcus aureus, one of the most important human pathogens, is the causative agent of several infectious diseases including sepsis, pneumonia, osteomyelitis, endocarditis and soft tissue infections. This pathogenicity is due to a multitude of virulence factors including several cell wall-anchored proteins (CWA). CWA proteins have modular structures with distinct domains binding different ligands. The majority of S. aureus strains express two CWA fibronectin (Fn)-binding adhesins FnBPA and FnBPB (Fn-binding proteins A and B), which are encoded by closely related genes. The N-terminus of FnBPA and FnBPB comprises an A ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: Available online 12 August 2020Source: Microbial PathogenesisAuthor(s): Ahmad Nasser, Taher Azimi, Soheila Ostadmohammadi, Samaneh Ostadmohammadi
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
ConclusionThe incidence of paediatric septic arthritis in New Zealand is concerningly high within M āori and Pacific populations. Future health interventions should consider environmental, socioeconomic, and microbiological trends in the burden of disease.
Source: International Orthopaedics - Category: Orthopaedics Source Type: research
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