Optimised lyophilisation-based method for different biomolecule single-extractions from the same rat brain sample: suitability for RNA and protein expression analyses after ischemic stroke

ConclusionsThe lyophilisation-based method for different biomolecule single-extractions from tissue powder aliquots, representing the same rat brain sample, is sample saving, contributes to the reduction principle in animal research, and allows coordinated analysis for accurate correlations between the transcriptome and proteome in stroke and other neuroscience research.
Source: Journal of Neuroscience Methods - Category: Neuroscience Source Type: research