FAM134B improves preadipocytes differentiation by enhancing mitophagy

In this study, we provide mechanistic links between FAM134B and adipocyte differentiation. Here, we found that adipocyte-specific FAM134B overexpression mice are obese and have increased white adipose tissue (WAT) mass. Serum tests showed that they developed high glucose level and severe insulin resistance. In addition, they also exhibited enhanced autophagy and reduced mitochondria amount, suggesting the function of FAM134B to promote autophagy in adipocytes. Overexpression of FAM134B in 3 T3-L1 preadipocytes promoted autophagy and differentiation, while the effect could be inhibited after treatment with autophagy inhibitors, 3-methyladenine (3-MA). Overexpression cells also showed an early reduction of mitochondria number, while its autophagy flux level increased fast from differentiation day 2. These findings indicate that FAM134B improves adipocytes differentiation through enhancing mitophagy.
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - Category: Lipidology Source Type: research