Modeling FXS with Mouse Neural Progenitors.
Modeling FXS with Mouse Neural Progenitors.
Methods Mol Biol. 2019;1942:71-78
Authors: Peteri UK, Castrén ML
Abstract
The neurosphere assay is a widely used method to culture neural precursor cells (NPCs), which include mixed populations of neural stem and progenitor cells, from the mammalian central nervous system. Fmr1-knockout (KO) mice generated to model fragile X syndrome (FXS) recapitulate the major phenotype of FXS. Neurosphere differentiation of cortical progenitors derived from brains of Fmr1-KO mice has been shown to reflect disordered mechanisms during cortical development in FXS in vivo. The cellular composition of neurospheres is heterogeneous, but robust FXS-specific alterations can be identified when culturing conditions are kept constant.
PMID: 30900176 [PubMed - indexed for MEDLINE]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Peteri UK, Castrén ML Tags: Methods Mol Biol Source Type: research