Qualification of a surface plasmon resonance assay to determine binding of IgG-type antibodies to complement component C1q

Publication date: Available online 21 August 2019Source: BiologicalsAuthor(s): Milan Jovic, Florian CymerAbstractInteraction of therapeutic antibodies with complement component C1q are frequently part of pharmaceutical characterization and production process comparability studies. Assays currently used to assess this interaction and/or the activation of the complement cascade are often cumbersome, time consuming or imprecise. We here report the further development, successful qualification and suitability evaluation of an SPR-based C1q binding assay for the characterization of IgG-C1q interactions. We evaluate different IgG subtypes and well-described mutants of IgG1. The assay offers a suitable alternative for extended characterization of interactions of IgG1 and IgG3 with complement component C1q.

Source: Biologicals - August 22, 2019 Category: Biology Source Type: research