A new role for the spleen: aggravation of the systemic inflammatory response in rats with severe acute pancreatitis.

A new role for the spleen: aggravation of the systemic inflammatory response in rats with severe acute pancreatitis. Am J Pathol. 2019 Aug 17;: Authors: Zhou R, Zhang J, Bu W, Zhang W, Duan B, Wang X, Yao L, Li Z, Li J Abstract Little is known about the role of the spleen in mediating systemic inflammatory responses in severe acute pancreatitis (SAP). Here, we investigated the role played by the spleen in rats after SAP induction. Splenectomy was performed at designated time points after SAP induction. Pancreatic tissue and serum samples were collected and subjected to histological, immunohistochemical, and immunological analyses. Following SAP induction, the splenic immune response was enhanced during SAP progression, as shown by the increased diameter of the splenic periarterial lymphatic sheath and the thickness of the splenic marginal zone. Rats with splenectomy developed acute pancreatitis more slowly than rats without splenectomy. Additionally, pancreatic tissues of rats with splenectomy contained lower levels of serum amylase, tumor necrosis factor-α, and interleukin-6 and exhibited less acinar cell death, leucocyte infiltration, and interstitial oedema than those of rats without splenectomy. Compared with splenectomy alone, cotreatment with splenectomy and the administration of splenic cells originating from a rat with SAP 12 h after induction increased systemic inflammation in SAP rats. Splenic factors exacerbated SAP-assoc...
Source: The American Journal of Pathology - Category: Pathology Authors: Tags: Am J Pathol Source Type: research