Geese not susceptible to virulent subgroup J avian leukosis virus isolated from chickens.

Geese not susceptible to virulent subgroup J avian leukosis virus isolated from chickens. Avian Pathol. 2019 Aug 20;:1-26 Authors: Mu X, Xu M, Zhu S, Xiao W, Shen X, Qin A Abstract To determine whether geese are infection of avian leukosis virus (ALV), 702 serum samples from domestic and foreign goose breeds were screened for p27 antigen as well as being inoculated into DF-1 cell cultures to isolate ALV. Although 5.6% of samples were positive for p27 antigen, reactivity appeared to be non-specific because no ALV was detected in the corresponding DF-1 cultures. To further determine whether geese are susceptible to ALV-J isolated from chickens, ALV-J strain JS09GY7 was artificially inoculated into 10-day-old goose embryos, with 1-day-old hatched goslings then screened for p27 antigen and the presence of ALV. In all cases, the results of both tests were negative. Liver tissues from the 1-day-old goslings were screened using a polymerase chain reaction-based assay, which failed to amplify ALV-J gene fragments from any of the samples. Further, no histopathological damage was observed in the liver tissues. ALV-J was further inoculated intraperitoneally into 1-day-old goslings, with cloacal swabs samples and plasma samples then collected every 5 days for 30 days. All samples were again negative for the presence of p27 antigen and ALV, and liver tissues from the challenged geese showed no histopathological damage and were negative for the pr...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research