Preparation Methods for Phospholipid Vesicle Arrays and Their Applications in Biological Analysis

Publication date: August 2019Source: Chinese Journal of Analytical Chemistry, Volume 47, Issue 8Author(s): Xue-Jing WANG, Wei MU, Xiao-Jun HANAbstractLipid vesicles, composed of cell membrane components, are not only used as an artificial cell model to mimic cell structure and functions, but also widely used in biological analysis. Liposome (phospholipid vesicle) array is a high throughput platform for protein screening and bio-sensing. This review focuses on the preparation methods of homogeneous small lipid vesicle array, heterogeneous small lipid vesicle array, and giant lipid vesicle array, as well as their applications in the antibiotics detection, enzymatic reactions, and the interactions between membrane proteins and lipid membranes. The advantages and disadvantages of the reported methods are summarized and the future development directions in this field are also proposed.Graphical abstractLipid vesicle array is a high throughput platform for protein screening and bio-sensing. This review focuses on the preparation methods of homogeneous/heterogeneous small lipid vesicle array, and giant lipid vesicle array, as well as their applications in antibiotics detection, enzymatic reactions, interactions between membrane proteins and lipid membranes, etc.

Source: Chinese Journal of Analytical Chemistry - August 21, 2019 Category: Chemistry Source Type: research