Detection of Tadalafil and Its Analogues in Health Wine and Oral Liquid by Microwell Lateral Flow Immunochromatography Assay

Publication date: August 2019Source: Chinese Journal of Analytical Chemistry, Volume 47, Issue 8Author(s): Ting-Ting ZOU, Jin-Yi YANG, Zhen-Lin XU, Hong WANG, Yuan-Ming SUN, Hong-Tao LEI, Xue-Cai TAN, Yu-Dong SHENAbstractTo monitor illegal addition of tadalafil and its analogues in health food, a new polyclonal antibody was prepared by immunizing New Zealand white rabbits with a common skeleton structure hapten combined with an unsaturated linear alkyl arm hapten strategy, which could simultaneously recognize tadalafil, amino tadalafil and N-Desmethyl tadalafil. A microwell lateral flow immune- chromatographic assay (mwLFIA) method combined with simple sample pretreatment was developed. The mwLFIA showed that the LOD for three tadalafil drugs was between 0.05 ng mL−1 and 0.30 ng mL−1, the cut-off values were between 50 ng mL−1 and 100 ng mL−1. Cross-reactivity (CR) results showed no interference from substances with similar structures, indicating a good specificity against tadalafil, amino tadalafil and N-Desmethyl tadalafil. The average recoveries of three drugs in spiked health wine and oral liquid ranged from 78.7% to 117.8% with an RSD of less than 15%, showing a good aggreement with the results of HPLC-MS/MS method. The developed mwLFIA strip was suitable for the rapid detection of tadalafil and its analogues in health wine and oral liquid samples.Graphical abstractA microwell lateral flow immunochromatographic assay (mwLFIA) for simultaneous determination of tad...
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research