A new perspective of lactatogenesis by isolated adipocytes

Publication date: Available online 20 August 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Filipy Borghi, Camila L. Morais, Carolina Silva, Priscila C. da Silva, Larissa Y. Ishizu, Gustavo T. Costa, Dora M. Grassi-KassisseAbstractIncreased adipose tissue mass exhibited greater capacity of glucose transformation in lactate, highlighting lactatogenesis as a crucial factor in body size. Classically, lactate produced by isolated adipocytes are expressed per million of cells and were never correlated with their size. Spontaneously hypertensive rats (SHR) have a lower body weight and smaller adipocytes when compared to Wistar-Kyoto. We evaluated basal lactate by epididymal 15−weeks-old isolated adipocytes of SHR, Wistar-Kyoto and Wistar. Basal lactate was similar when expressed by one million cells. However, SHR adipocytes were smaller, so we adjusted the results by cell volume and SHR showed higher basal lactate production which was directly endorsed by hyperlactatemia in the presented conditions. Thereby, we suggest a new perspective on lactatogenesis analysis by adipocytes, which could be linked to the receptors density and associate enzymes. Moreover, we showed that the thin and hypertensive rats can be hyperlactemic in fasting conditions.

Source: Molecular and Cellular Endocrinology - August 21, 2019 Category: Endocrinology Source Type: research