Generation of a CFTR knock-in reporter cell line (MHHi006-A-1) from a human induced pluripotent stem cell line

Publication date: Available online 20 August 2019Source: Stem Cell ResearchAuthor(s): Lena Engels, Ruth Olmer, Jeanne de la Roche, Gudrun Göhring, Saskia Ulrich, Ralf Haller, Ulrich Martin, Sylvia MerkertAbstractCFTR encodes for a chloride ion channel expressed primarily in secretory epithelia in the airways, intestine, liver and other tissues. Mutations in the CFTR gene have been identified in people suffering from Cystic Fibrosis. Here, we established a CFTR knock-in reporter cell line from a human iPSC line (MHHi006-A) using TALEN technology. The reporter enables the monitoring and optimization of the differentiation of pluripotent stem cells into CFTR expressing epithelia on a single cell level, as well as the enrichment of CFTR positive cells, which represent an excellent tool for Cystic Fibrosis disease modelling, drug screening and ultimately cellular therapies.
Source: Stem Cell Research - Category: Stem Cells Source Type: research