A new approach to the production of a biovital skin graft based on human acellular dermal matrix produced in ‐house, in vitro revitalized internally by human fibroblasts and keratinocytes on the surface

AbstractPatients with extensive and deep burns who do not have enough donor sites for autologous skin grafts require alternative treatment methods. Tissue engineering is a useful tool to solve this problem. The aim of this study was to find the optimal method for the production of a biovital skin substitute based on acellular dermal matrix (ADM) and in vitro cultured fibroblasts and keratinocytes. In this work, nine methods of ADM production were assessed. The proposed methods are based on the use of the following enzymes: Dispase II, collagenase I/ethylenediaminetetraacetic acid (EDTA), collagenase II/EDTA, and mechanical perforation using DermaRoller and mesh dermatome. The obtained ADMs were examined (both on the side of the basement membrane and on the “cut‐off” side) by means of scanning electron microscopy, immunohistochemistry tests and strength tests. ADM was revitalized with human fibroblasts and keratinocytes. The ability of in‐depth revitalization of cultured fibroblasts and their ability to secrete collagen IV was examined. The obt ained results indicate that the optimal method of production of live skin substitutes is the colonization of autologous fibroblasts and keratinocytes on the scaffold obtained using two‐step incubation method: Trypsin/EDTA and dispase II.
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - Category: Materials Science Authors: Tags: ORIGINAL RESEARCH REPORT Source Type: research

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ConclusionThe presence of wellness support was associated with better outcomes, suggesting the value of initiatives to manage workload and support the well-being of surgical resident physicians.
Source: The American Journal of Surgery - Category: Surgery Source Type: research
Sometime during the 90s, I listened to an audio program that explained the differences between matchers and mismatchers.Matchers are “yes, and” people who see the upsides of an idea more prominently than the downsides. If you share a new idea with them, they’ll see how the idea could work and may offer suggestions for what else you could do to make it even better.Mismatchers are “wait, but” people who see the downsides of an idea more prominently than the upsides. If you share a new idea with them, they see what could go wrong and may point out the risks and drawbacks.I’m a matcher. I fi...
Source: Steve Pavlina's Personal Development Blog - Category: Psychiatry & Psychology Authors: Tags: Productivity Source Type: blogs
Publication date: Available online 20 October 2019Source: BurnsAuthor(s): Mickaël Tobalem, Reto Wettstein, Elizabeth Tschanz, Jan Plock, Nicole Lindenblatt, Yves Harder, Farid RezaeianAbstractIntroductionThe burn comb model is a well-established model for studying secondary burn progression. It creates four rectangular burn surfaces intercalated by three unburned zones prone to secondary burn progression. While burn progression is a tri-dimensional phenomenon, of which the vertical extension from the superficial to deeper tissue layer is clinically most relevant, the models initial focus was mainly on the horizontal s...
Source: Burns - Category: Dermatology Source Type: research
Publication date: Available online 20 October 2019Source: BurnsAuthor(s): Luiz Philipe Molina Vana, Cláudia Naves Battlehner, Marcelo Alves Ferreira, Elia Garcia Caldini, Rolf Gemperli, Nivaldo AlonsoAbstractThe advent of dermal regeneration templates has fostered major advances in the treatment of acute burns and their sequelae, in the last three decades. Both data on morphological aspects of the newly-formed tissue, and clinical trials comparing different templates, are few. The goal of this study was to prospectively analyze the outcome of randomized patients treated with two of the existing templates, followed b...
Source: Burns - Category: Dermatology Source Type: research
We describe the case of an 11-year-old female presenting with Cushingoid features in the setting of a left-sided flank mass. Her presentation and evaluation suggested a paraneoplastic ectopic ACTH syndrome. She underwent open left radical nephrectomy and final pathology confirming a high-grade NET with nodal metastasis. Although exceedingly rare, ACTH-secreting tumors of the kidney can cause significant morbidity and mortality and so we recommend it be included in the differential diagnosis of pediatric renal masses. PMID: 31629447 [PubMed - in process]
Source: Canadian Journal of Urology - Category: Urology & Nephrology Tags: Can J Urol Source Type: research
By BRYAN CARMODY Recently, I was on The Accad and Koka Report to share my opinions on USMLE Step 1 scoring policy. (If you’re interested, you can listen to the episode on the show website or iTunes.) Most of the topics we discussed were ones I’ve already dissected on this site. But there was an interesting moment in the show, right around the 37:30 mark, that raises an important point that is worthy of further analysis. __ ANISH: There’s also the fact that nobody is twisting the arms of program directors to use [USMLE Step 1] scores, correct? Even in an era when y...
Source: The Health Care Blog - Category: Consumer Health News Authors: Tags: Medical Education Medical Practice Physicians Bryan Carmody Step 1 USMLE Step 1 Source Type: blogs
A new study published inJournal of Clinical Endocrinology and Metabolism suggests exercising before breakfast burns more fat and improves weight loss.CNN Health
Source: Society for Endocrinology - Category: Endocrinology Source Type: news
CONCLUSIONS: Due to the presence of hypothermia on admission, and to the fact that the average temperature does not exceed 36°C until at least 16hours after the burn, nurses must know and promptly administer adequate reheating measures to improve chances of survival in major burns. PMID: 31629638 [PubMed - as supplied by publisher]
Source: Enfermeria Intensiva - Category: Nursing Tags: Enferm Intensiva Source Type: research
Vacuum sealing drainage (VSD) vacuum suction in the hospital burn department with an ozone bath dressing is used to enhance the rate of arterial ulcer healing in lower extremity arterial ulcers.
Source: Journal of Vascular Surgery - Category: Surgery Authors: Source Type: research
ConclusionDeposition of fibrosis-related proteins after severe burn is contributors to decreased small intestinal motility.
Source: Journal of Gastrointestinal Surgery - Category: Surgery Source Type: research
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