Differentiation of live and dead Mycobacterium tuberculosis complex in meat samples using PMA qPCR.

Differentiation of live and dead Mycobacterium tuberculosis complex in meat samples using PMA qPCR. Food Microbiol. 2019 Dec;84:103275 Authors: Dorn-In S, Gareis M, Schwaiger K Abstract The causative agents of zoonotic bovine tuberculosis (bTB), Mycobacterium bovis and M. caprae, are members of the M. tuberculosis complex (MTC). Wildlife such as red deer infected with bTB are often without pathological findings, thus meat thereof may be classified as safe for human consumption. The culturing of MTC is time consuming and inappropriate to be applied with fresh meat and food. Therefore, a rapid method "PMA qPCR" to differentiate living and dead cells of MTC was developed in this study. By treating with 50 μM PMA™ dye, dead M. bovis BCG (≤104 cells/ml meat suspension) could be completely discriminated and was not detected by specific MTC PCR. The limit of detection of MTC without treatment with PMA™ dye was 10 cells/ml. All 50 venison samples obtained for field study purposes were negative for MTC. However, 40% were slightly PCR positive for non-TBC mycobacteria. By culturing using selective enrichment, one single colony of M. avium was isolated. This is the first report on the isolation of M. avium from venison. Considering the difficulties of diagnosing mycobacteria in various matrices, the developed PMA qPCR is applicable for the differentiation of dead and living cells of MTC in meat samples. PMID: 31421753 [PubMed ...
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research

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Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
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Source: Journal of Comparative Pathology - Category: Pathology Source Type: research
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Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
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Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: 20.070 Source Type: research
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Source: BMC Infectious Diseases - Category: Infectious Diseases Authors: Tags: Research article Source Type: research
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