Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis.

Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis. Methods Mol Biol. 2019;1930:51-57 Authors: Prasannan P, Verma NK Abstract The ability of activated T-lymphocytes to transmigrate toward certain chemokine is one of their characteristic functional properties. Here, we provide step-wise details about an in vitro technique to quantify the kinetics of chemotactic behavior of LFA-1-stimulated T-lymphocytes. The method described herein utilizes a noninvasive electrical impedance-based detection system to monitor T-cell chemotaxis in real-time. PMID: 30610598 [PubMed - indexed for MEDLINE]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research